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The gene DIS2S1 is essential in Saccharomyces cerevisiae and is involved in glycogen phosphorylase activation.

作者信息

Clotet J, Posas F, Casamayor A, Schaaff-Gerstenschläger I, Ariño J

机构信息

Department de Bioquímica i Biología Molecular, Facultat de Veterinaria, Universitat Autònoma de Barcelona, Spain.

出版信息

Curr Genet. 1991 May;19(5):339-42. doi: 10.1007/BF00309593.

DOI:10.1007/BF00309593
PMID:1913873
Abstract

S. cerevisiae gene DIS2S1, which codes for a protein very similar to the catalytic subunit of mammalian protein phosphatase 1, was disrupted "in vitro". Diploid yeast cells were transformed and sporulated. Tetrad analysis demonstrated that disruption of DIS2S1 is lethal for the cell. Glycogen phosphorylase alpha and glycogen synthase activity ratio were measured in diploids carrying a disrupted allele of the gene. Phosphorylase was dramatically activated in mutant cells but, under the same conditions, glycogen synthase activity was essentially identical in both mutant and wild-type cells.

摘要

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本文引用的文献

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A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.一种将DNA限制性内切酶片段放射性标记至高比活度的技术。
Anal Biochem. 1983 Jul 1;132(1):6-13. doi: 10.1016/0003-2697(83)90418-9.
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One-step gene disruption in yeast.酵母中的一步基因破坏
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Characterization of phosphoprotein phosphatases and phosphorylase phosphatase from yeast.酵母中磷酸蛋白磷酸酶和磷酸化酶磷酸酶的特性分析
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Modulation of yeast alkaline cation tolerance by Ypi1 requires calcineurin.Ypi1 通过钙调神经磷酸酶调节酵母碱性阳离子耐受性。
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Alkali metal cation transport and homeostasis in yeasts.酵母中的碱金属阳离子运输和稳态。
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Evidence for the involvement of the Glc7-Reg1 phosphatase and the Snf1-Snf4 kinase in the regulation of INO1 transcription in Saccharomyces cerevisiae.关于Glc7-Reg1磷酸酶和Snf1-Snf4激酶参与酿酒酵母中INO1转录调控的证据。
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The yeast halotolerance determinant Hal3p is an inhibitory subunit of the Ppz1p Ser/Thr protein phosphatase.酵母耐盐性决定因子Hal3p是Ppz1p丝氨酸/苏氨酸蛋白磷酸酶的一个抑制亚基。
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The mutant type 1 protein phosphatase encoded by glc7-1 from Saccharomyces cerevisiae fails to interact productively with the GAC1-encoded regulatory subunit.酿酒酵母中由glc7-1编码的突变型1型蛋白磷酸酶无法与GAC1编码的调节亚基进行有效的相互作用。
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A rapid filter paper assay for UDPglucose-glycogen glucosyltransferase, including an improved biosynthesis of UDP-14C-glucose.一种用于UDP葡萄糖-糖原葡萄糖基转移酶的快速滤纸测定法，包括改进的UDP-14C-葡萄糖生物合成。
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8
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Eur J Biochem. 1988 Jun 15;174(3):561-7. doi: 10.1111/j.1432-1033.1988.tb14135.x.
9
Molecular analysis of GPH1, the gene encoding glycogen phosphorylase in Saccharomyces cerevisiae.酿酒酵母中编码糖原磷酸化酶的基因GPH1的分子分析。
Mol Cell Biol. 1989 Apr;9(4):1659-66. doi: 10.1128/mcb.9.4.1659-1666.1989.
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One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution.大肠杆菌感受态细胞的一步制备法：在同一溶液中进行细菌细胞的转化与保存。
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