Ji Peng-Zhang, Jiang Hui-Bing, Huang Xing-Qi, Zhang Jun, Liang Min-Zhi, Wang Ping-Sheng
College of Life Sciences, Yunnan University, Kunming 650091, China.
Yi Chuan. 2009 Jan;31(1):101-8. doi: 10.3724/sp.j.1005.2009.00101.
This study was conducted to evaluate the genetic diversity within and among the plants of four ancient tea gardens and two tableland tea gardens form Yunnan Province, China by AFLP technique. The percentage of polymorphic loci (P) of the plants from six tea gardens was 92.31%. The genetic diversity within the six gardens demonstrated by Nei cents genetic diversity (He) was estimated to be 0.1366, while Shannon indices (Ho) were 0.2323. The percentage of polymorphic loci of the four ancient tea populations was 45.55% on average, with a range of 36.44% (Mengsong) to 59.11% (Mengla). But the percentages of polymorphic loci of the plants from two tableland gardens were 13.77% (Yunkang 10) and 24.2% (Menghai Daye), respectively. There was a great genetic difference between ancient tea gardens and tableland tea gardens. The genetic diversity among the plants of the ancient tea garden was higher than those of the sexual tableland tea garden and the clone tableland tea garden based on P valve. The four ancient tea gardens and two tableland gardens could be differentiated with AFLP markers. The results show that AFLP marker is an effective tool in the discrimination of tea germplasm, as well as sundried green tea.
本研究采用AFLP技术，对中国云南省四个古茶园和两个山地茶园的茶树植株内及植株间的遗传多样性进行评估。六个茶园植株的多态位点百分比（P）为92.31%。通过Nei氏遗传多样性（He）评估的六个茶园内的遗传多样性估计为0.1366，而香农指数（Ho）为0.2323。四个古茶群体的多态位点百分比平均为45.55%，范围为36.44%（勐宋）至59.11%（勐腊）。但两个山地茶园植株的多态位点百分比分别为13.77%（云抗10号）和24.2%（勐海大叶）。古茶园和山地茶园之间存在很大的遗传差异。基于P值，古茶园植株间的遗传多样性高于有性山地茶园和无性系山地茶园。四个古茶园和两个山地茶园可用AFLP标记进行区分。结果表明，AFLP标记是鉴别茶树种质以及晒青绿茶的有效工具。
