Zuckerman S H, Douglas S D
Immunology. 1977 Mar;32(3):247-53.
The effect of cholera exotoxin and aminophylline on Fc receptors in a murine lymphoid-cell line and in rabbit pulmonary alveolar macrophages has been investigated. Although both agents elevated intracellular cyclic AMP levels in macrophages and lymphoid cells, the effects on Fc receptor expression were distinct. Cholera toxin at 10 microng/ml reversibly inhibited Fc-receptor activity in the murine lymphoid cell line. In contrast, cholera toxin at 10 microng/ml or 0-01 microng/ml was ineffective in altering pulmonary alveolar macrophage receptor expression. Fc receptor activity on the macrophage was reduced by 20-30 per cent following incubation with aminophylline, (10(-3)M) from 0-6 h. There was no direct correlation between Fc-receptor activity and cyclic AMP levels in the cells studied. The differential susceptibility of these lymphoid and phagocytic cell populations to cholera toxin and also toward aminophylline suggests that there may be fundamental differences in topography on the membrane surface, or in the intracellular regulation of Fc receptors between lymphoid and phagocytic cells.
已研究了霍乱毒素和氨茶碱对小鼠淋巴细胞系及兔肺泡巨噬细胞中Fc受体的作用。尽管这两种药物均可提高巨噬细胞和淋巴细胞内的环磷酸腺苷（cAMP）水平，但对Fc受体表达的影响却截然不同。10微克/毫升的霍乱毒素可可逆性抑制小鼠淋巴细胞系中的Fc受体活性。相比之下，10微克/毫升或0.01微克/毫升的霍乱毒素对改变肺泡巨噬细胞受体表达无效。巨噬细胞与10⁻³M氨茶碱孵育0至6小时后，其Fc受体活性降低了20%至30%。在所研究的细胞中，Fc受体活性与cAMP水平之间无直接相关性。这些淋巴细胞和吞噬细胞群体对霍乱毒素以及氨茶碱的不同敏感性表明，淋巴细胞和吞噬细胞在膜表面拓扑结构或Fc受体的细胞内调节方面可能存在根本差异。
