抗原被体外经抗血清处理过的脾细胞吸附。
The adsorption of antigen by spleen cells previously treated with antiserum in vitro.
作者信息
BOYDEN S V, SORKIN E
出版信息
Immunology. 1960 Jul;3(3):272-83.
Rabbit spleen cells, which have been treated with certain rabbit or guinea-pig antisera (e.g. against human serum albumin) and washed, are capable of specifically adsorbing radio-isotope labelled antigen. The antibody responsible for this effect appears to be distinct from the main precipitating antibody in the serum and the term `cytophilic antibody' has been suggested. The activity of the cytophilic antibody is not destroyed by heating at 56° C. for half an hour. The cytophilic antibody is not adsorbed by red cells. Spleen cells which have been treated with methanol before treatment with antiserum take up less antigen than non-methanol-treated cells tested in the same way. However, the addition of fresh normal spleen cells to the methanol-antibody-treated cells before the addition of antigen increased the uptake of the latter.
用某些兔或豚鼠抗血清（如抗人血清白蛋白）处理并洗涤过的兔脾细胞，能够特异性吸附放射性同位素标记的抗原。引起这种效应的抗体似乎与血清中的主要沉淀抗体不同，有人提出了“嗜细胞抗体”这一术语。嗜细胞抗体的活性不会因在56℃加热半小时而被破坏。嗜细胞抗体不会被红细胞吸附。在用抗血清处理之前先用甲醇处理过的脾细胞，与以同样方式测试的未用甲醇处理的细胞相比，摄取的抗原较少。然而，在加入抗原之前，向经甲醇-抗体处理的细胞中加入新鲜的正常脾细胞，会增加后者对抗原的摄取。
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