Popescu Bogdan F Gh, Robinson Christopher A, Rajput Alex, Rajput Ali H, Harder Sheri L, Nichol Helen
Department of Anatomy and Cell Biology, College of Medicine, University of Saskatchewan, Saskatoon, SK, Canada.
Cerebellum. 2009 Jun;8(2):74-9. doi: 10.1007/s12311-008-0091-3. Epub 2009 Jan 13.
Synchrotron rapid-scanning X-ray fluorescence (RS-XRF) is employed for the first time to simultaneously map iron, copper, and zinc in the normal cerebellum. The cerebellum is a major repository of metals that are essential to normal function. Therefore, mapping the normal metal distribution is an important first step towards understanding how multiple metals may induce oxidative damage, protein aggregation, and neurotoxicity leading to cerebellar degeneration in a wide range of diseases. We found that cerebellar white and grey matter could be sharply defined based upon the unique metal content of each region. The dentate nucleus was particularly metal-rich with copper localized to the periphery and iron and zinc abundant centrally. We discuss how RS-XRF metal mapping in the normal brain may yield important clues to the mechanisms of degeneration in the dentate nucleus.
同步加速器快速扫描X射线荧光（RS-XRF）首次被用于同时绘制正常小脑中铁、铜和锌的分布图。小脑是对正常功能至关重要的金属的主要储存库。因此，绘制正常金属分布是理解多种金属如何在广泛疾病中诱导氧化损伤、蛋白质聚集和神经毒性从而导致小脑变性的重要第一步。我们发现，小脑白质和灰质可以根据每个区域独特的金属含量清晰界定。齿状核金属含量特别丰富，铜集中在外围，铁和锌在中央大量存在。我们讨论了正常大脑中的RS-XRF金属图谱如何可能为齿状核变性机制提供重要线索。
