Kaji T, Hayashi T, Miezi N, Kaga K, Ejiri N, Sakuragawa N
Department of Clinical Laboratory Medicine, Faculty of Medicine, Toyama Medical and Pharmaceutical University, Japan.
Chem Pharm Bull (Tokyo). 1991 May;39(5):1312-4. doi: 10.1248/cpb.39.1312.
We investigated the effect of a hot water extract from Gardenia fruit (Gardenia jasminoides Ellis) (GFE), which has a stimulatory effect on endothelial cell proliferation, on the proliferation of A10 cells, an established cell line of vascular smooth muscle cell from murine aorta in a culture system. GFE did not change the number of A10 cells after a 48 h culture. GFE significantly increased the incorporation of [3H]thymidine and [14C]leucine into the acid-soluble fraction of bovine aortic endothelial cell layers, but significantly decreased that of A10 cells. These results suggested that GFE stimulates the proliferation of endothelial cells but not of A10 cells. In the endothelial cell culture, GFE significantly increased the accumulation of basic fibroblast growth factor, which is an autocrine for endothelial cell proliferation in medium and low-affinity (glycosaminoglycans-binding) fractions, while A10 cells did not produce a significant amount of the factor. Since it is postulated that a selective stimulation of endothelial cell proliferation by increasing the production of basic fibroblast growth factor is appropriate for prevention of arteriosclerosis and thrombosis, GFE may contain a beneficial component as a useful drug.
我们研究了栀子果实（Gardenia jasminoides Ellis）热水提取物（GFE）对A10细胞增殖的影响，A10细胞是一种来自小鼠主动脉的血管平滑肌细胞系，在培养体系中已确立。在48小时培养后，GFE未改变A10细胞的数量。GFE显著增加了[3H]胸苷和[14C]亮氨酸掺入牛主动脉内皮细胞层酸溶性部分的量，但显著降低了A10细胞的掺入量。这些结果表明，GFE刺激内皮细胞增殖，但不刺激A10细胞增殖。在内皮细胞培养中，GFE显著增加了碱性成纤维细胞生长因子的积累，碱性成纤维细胞生长因子是内皮细胞增殖的自分泌因子，存在于中、低亲和力（与糖胺聚糖结合）部分，而A10细胞未产生大量该因子。由于推测通过增加碱性成纤维细胞生长因子的产生来选择性刺激内皮细胞增殖适用于预防动脉硬化和血栓形成，GFE可能含有作为有用药物的有益成分。
