Kaji T, Miezi N, Kaga K, Ejiri N, Sakuragawa N
Department of Clinical Laboratory Medicine, Faculty of Medicine, Toyama Medical and Pharmaceutical University, Japan.
Planta Med. 1990 Aug;56(4):353-6. doi: 10.1055/s-2006-960980.
We previously reported that Gardenia fruit extract had a stimulatory effect on fibrolysis in vitro. In the present study, we describe that the hot water extract from Gardenia fruit (GFE) stimulates the proliferation of cultured endothelial cells from bovine aorta. The cells were cultured in RPMI1640 medium supplemented 10% fetal bovine serum for up to 96 h in the presence of GFE (100 micrograms/ml and below). GFE at 25 and 50 micrograms/ml significantly increased the cell number after 48 h and above of the treatment. The incorporations of [3H]thymidine and [14C]leucine were significantly increased by 50 micrograms/ml GFE. The GFE stimulation of the proliferation was completely inhibited by 1 microM cycloheximide. The stimulatory activity of GFE was recognized in the low relative molecular mass (less than or equal to 10,000) fraction. In conclusion, it was demonstrated that a low molecular mass component of GFE stimulated endothelial cell proliferation and an increased protein synthesis was a essential component of this response. GFE may contain a useful compound to stimulate the proliferation of endothelial cells.
我们之前报道过栀子果实提取物在体外对纤维蛋白溶解有刺激作用。在本研究中，我们描述了栀子果实热水提取物（GFE）能刺激培养的牛主动脉内皮细胞增殖。细胞在补充有10%胎牛血清的RPMI1640培养基中培养，在存在GFE（100微克/毫升及以下）的情况下培养长达96小时。25和50微克/毫升的GFE在处理48小时及以上后显著增加了细胞数量。50微克/毫升的GFE显著增加了[3H]胸腺嘧啶核苷和[14C]亮氨酸的掺入量。GFE对增殖的刺激被1微摩尔的环己酰亚胺完全抑制。GFE的刺激活性在低相对分子质量（小于或等于10,000）组分中被识别。总之，已证明GFE的低分子质量成分刺激内皮细胞增殖，蛋白质合成增加是该反应的一个重要组成部分。GFE可能含有一种有用的化合物来刺激内皮细胞增殖。
