Kaji T, Kaga K, Miezi N, Hayashi T, Ejiri N, Sakuragawa N
Department of Clinical Laboratory Medicine, Faculty of Medicine, Toyama Medical and Pharmaceutical, Japan.
Chem Pharm Bull (Tokyo). 1990 Sep;38(9):2494-7. doi: 10.1248/cpb.38.2494.
To investigate the possible mechanism of the stimulatory effect of a hot water extract from Artemisia leaf (Artemisia princeps PANPANINI) (AFE) on the proliferation of endothelial cells, cells from bovine aorta were cultured for 72 h in RPMI1640 medium supplemented with 10% fetal calf serum in the presence of 5 micrograms/ml AFE. The AFE treatment significantly increased the cell number after culture, while in the presence of 10 micrograms/ml unfractionated heparin, AFE conversely decreased it. This implied that AFE enhanced the cell growth promotion by basic fibroblast growth factor (bFGF). The accumulation of bFGF was significantly increased in the culture medium, in the low-affinity (glycosaminoglycans-binding) fraction, and in the cell extract fraction, but was unchanged in the high-affinity (receptor-binding) fraction. The contents of [35S]sulfate-labeled glycosaminoglycans in both cell layer and the medium were not increased by AFE treatment. The proliferation of A10 cells, an established cell line of smooth muscle cells from murine aorta, was not stimulated by AFE. A10 cells did not produce a significant amount of bFGF in the presence or absence of AFE. Thus, the production of bFGF was considered to be involved in AFE stimulation of cell proliferation. In conclusion, it was suggested that AFE stimulated endothelial cell proliferation by increasing the production of bFGF rather than by an increase in the number of bFGF receptors and the content of glycosaminoglycans in the cell layer. The enhanced reserve of bFGF in the low-affinity fraction of cell layer and in the medium would cause the AFE-stimulated proliferation of endothelial cells.
为研究艾叶（Artemisia princeps PANPANINI）热水提取物（AFE）对内皮细胞增殖的刺激作用的可能机制，将牛主动脉细胞在补充有10%胎牛血清的RPMI1640培养基中于5微克/毫升AFE存在的情况下培养72小时。AFE处理显著增加了培养后的细胞数量，而在10微克/毫升未分级肝素存在的情况下，AFE反而使其减少。这表明AFE通过碱性成纤维细胞生长因子（bFGF）增强了细胞生长促进作用。bFGF在培养基、低亲和力（糖胺聚糖结合）部分和细胞提取物部分中的积累显著增加，但在高亲和力（受体结合）部分中未发生变化。AFE处理未增加细胞层和培养基中[35S]硫酸盐标记的糖胺聚糖的含量。AFE未刺激A10细胞（一种来自小鼠主动脉的平滑肌细胞系）的增殖。无论有无AFE，A10细胞均不产生大量bFGF。因此，bFGF的产生被认为与AFE对细胞增殖的刺激有关。总之，提示AFE通过增加bFGF的产生而非通过增加bFGF受体数量和细胞层中糖胺聚糖的含量来刺激内皮细胞增殖。细胞层低亲和力部分和培养基中bFGF储备的增加将导致AFE刺激的内皮细胞增殖。
