[自由基对单层培养兔关节软骨细胞的影响]
[The effect of free radicals on the rabbit articular chondrocytes in monolayer culture].
作者信息
Kang Y
机构信息
Department of Pathology, Xian Medical University.
出版信息
Zhonghua Bing Li Xue Za Zhi. 1991 Jun;20(2):100-2.
By using monolayer culture technique, the effect of endogenous free radicals on rabbit articular chondrocytes was studied. The free radicals were provided through the action of xanthine oxidase (XO) on xanthine (X). The amount of DNA in chondrocytes was measured with ethidium bromide method for direct fluorometric estimation of DNA. The synthesis of proteoglycan (PG) was assayed through 35S-Na2SO4 incorporation followed by inverted microscopy, scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The results indicated that chondrocytes could be damaged by endogenous free radicals generated by the action of XO on X, and these free radicals may be related to the pathogenesis of cartilage aging, degeneration, degenerative joint disease and arthritis.
采用单层培养技术，研究内源性自由基对兔关节软骨细胞的影响。自由基通过黄嘌呤氧化酶（XO）作用于黄嘌呤（X）产生。用溴化乙锭法直接荧光测定软骨细胞中的DNA含量。通过掺入35S-Na2SO4，然后用倒置显微镜、扫描电子显微镜（SEM）和透射电子显微镜（TEM）检测蛋白聚糖（PG）的合成。结果表明，XO作用于X产生的内源性自由基可损伤软骨细胞，这些自由基可能与软骨老化、退变、退行性关节病和关节炎的发病机制有关。
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