四种益生菌菌株对小鼠实验性结肠炎的治疗作用
Therapeutic effects of four strains of probiotics on experimental colitis in mice.
作者信息
Chen Lin Lin, Wang Xue Hong, Cui Yi, Lian Guang Hui, Zhang Jie, Ouyang Chun Hui, Lu Fang Gen
机构信息
Department of Gastroenterology, The Second Xiangya Hospital of Central South University, Changsha, Hunan Province, China.
出版信息
World J Gastroenterol. 2009 Jan 21;15(3):321-7. doi: 10.3748/wjg.15.321.
AIM
To investigate the therapeutic effects of four strains of probiotics (E. feacalis, L. acidophilus, C. butyricum and B. adolescentis) on dextran sulphate sodium (DSS)-induced experimental colitis in Balb/c mice.
METHODS
Eighty Balb/c mice were randomly divided into 8 groups. Weight-loss, fecal character, fecal occult blood and hematochezia were recorded daily. Disease activity index (DAI) scores were also evaluated everyday. Length of colon was measured and histological scores were evaluated on the 13th day. Myeloperoxidase (MPO) activity was detected. Interleukin-1 (IL-1) and IL-4 expression was detected by ELISA and RT-PCR.
RESULTS
The four strains of probiotics relieved the inflammatory condition of DSS-induced experimental colitis in mice. Weight loss was slowed down in all probiotics-treated mice. Even weight gain was observed by the end of probiotics treatment. The DAI and histological scores of probiotics-treated mice were lower than those of mice in the control group (1.9 +/- 0.2 vs 8.6 +/- 0.4, P < 0.05 for E. faecalis). The length of colon of probiotics-treated mice was longer than that of mice in the control group (10.3 +/- 0.34 vs 8.65 +/- 0.77, P < 0.05 for E. faecalis). The four strains of probiotics decreased the MP activity and the IL-1 expression, but increased the IL-4 expression. E. faecalis had a better effect on DSS-induced experimental colitis in mice than the other three strains.
CONCLUSION
The four strains of probiotics have beneficial effects on experimental colitis in mice. E. faecalis has a better effect on DSS-induced experimental colitis in mice than the other three strains. Supplement of probiotics provides a new therapy for UC.
目的
研究四种益生菌菌株（粪肠球菌、嗜酸乳杆菌、丁酸梭菌和青春双歧杆菌）对葡聚糖硫酸钠（DSS）诱导的Balb/c小鼠实验性结肠炎的治疗作用。
方法
将80只Balb/c小鼠随机分为8组。每天记录体重减轻、粪便性状、粪便潜血和便血情况。每天还评估疾病活动指数（DAI）评分。在第13天测量结肠长度并评估组织学评分。检测髓过氧化物酶（MPO）活性。通过酶联免疫吸附测定（ELISA）和逆转录聚合酶链反应（RT-PCR）检测白细胞介素-1（IL-1）和IL-4表达。
结果
四种益生菌菌株缓解了DSS诱导的小鼠实验性结肠炎的炎症状况。所有用益生菌治疗的小鼠体重减轻均减缓。在益生菌治疗结束时甚至观察到体重增加。用益生菌治疗的小鼠的DAI和组织学评分低于对照组小鼠（粪肠球菌组为1.9±0.2对8.6±0.4，P<0.05）。用益生菌治疗的小鼠的结肠长度长于对照组小鼠（粪肠球菌组为10.3±0.34对8.65±0.77，P<0.05）。四种益生菌菌株降低了MP活性和IL-1表达，但增加了IL-4表达。粪肠球菌对DSS诱导的小鼠实验性结肠炎的治疗效果优于其他三种菌株。
结论
四种益生菌菌株对小鼠实验性结肠炎有有益作用。粪肠球菌对DSS诱导的小鼠实验性结肠炎的治疗效果优于其他三种菌株。补充益生菌为溃疡性结肠炎提供了一种新的治疗方法。
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