Pan T, Guo H Y, Zhang H, Liu A P, Wang X X, Ren F Z
Beijing Laboratory for Food Quality and Safety, and Key Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University, P.O. Box 303, No. 17 Tsinghua East Road, Beijing 100083, China P.R.
Beijing Higher Institution Engineering Research Center of Animal Product, China Agricultural University, No. 17 Tsinghua East Road, Beijing 100083, China P.R.
Benef Microbes. 2014 Sep;5(3):315-22. doi: 10.3920/BM2013.0041.
The aim of this study was to investigate the alleviating effect of Lactobacillus paracasei subsp. paracasei LC-01 (LC-01) on the murine model of colitis induced by dextran sulphate sodium (DSS). 50 pathogen-free, 6-week-old male BALB/c mice were divided randomly into 5 groups, including a control group and four DSS-LC-01-treated groups (DSS, DSS-106, DSS-108, and DSS-1010 with 0, 1×106, 1×108 and 1×1010 cfu/ml LC-01, respectively). To test the effectiveness of LC-01 as a prophylactic it was administered for 7 days before the onset of the disease in DSS-LC-01-treated mice. After 7 days, colitis was induced by administration of 2.5% (w/v) DSS in drinking water for a further 7 days. The disease activity index (DAI), histological score, myeloperoxidase (MPO) activity and the level of the pro-inflammatory cytokines interleukin-1β (IL-1β) and tumour necrosis factor α (TNF-α) were measured. DAI, histological scores and MPO activity of mice treated with a medium or high dose of LC-01 were significantly lower compared to a low-dose of LC-01 and DSS treatment alone (P<0.05). Colon length shortening could be prevented with increasing dose of LC-01. In addition, the levels of IL-1β and TNF-α were suppressed significantly by treatment with a medium and high dose of LC-01. However, no significant difference in the indices mentioned above were observed between a low dose of LC-01 and treatment with DSS alone (P≯0.05). An appropriate dose of LC-01 can prevent intestinal damage in mice with DSS-induced colitis. The expression of inflammatory cytokines related to pathogenesis of DSS-induced colitis decreased following treatment with LC-01.
本研究旨在探讨副干酪乳杆菌副干酪亚种LC-01（LC-01）对葡聚糖硫酸钠（DSS）诱导的小鼠结肠炎模型的缓解作用。将50只6周龄无特定病原体的雄性BALB/c小鼠随机分为5组，包括1个对照组和4个DSS-LC-01处理组（DSS、DSS-106、DSS-108和DSS-1010，分别含有0、1×106、1×108和1×1010 cfu/ml的LC-01）。为了测试LC-01作为预防剂的有效性，在DSS-LC-01处理组小鼠疾病发作前7天给予该菌。7天后，通过在饮用水中给予2.5%（w/v）DSS再持续7天来诱导结肠炎。测量疾病活动指数（DAI）、组织学评分、髓过氧化物酶（MPO）活性以及促炎细胞因子白细胞介素-1β（IL-1β）和肿瘤坏死因子α（TNF-α）的水平。与低剂量LC-01组和单独DSS处理组相比，中高剂量LC-01处理组小鼠的DAI、组织学评分和MPO活性显著降低（P<0.05）。随着LC-01剂量增加，结肠长度缩短可得到预防。此外，中高剂量LC-01处理可显著抑制IL-1β和TNF-α水平。然而，低剂量LC-01组与单独DSS处理组之间在上述指标上未观察到显著差异（P>0.05）。适当剂量的LC-01可预防DSS诱导的小鼠结肠炎肠道损伤。LC-01处理后，与DSS诱导的结肠炎发病机制相关的炎性细胞因子表达降低。
