Vasil'eva I O, Neguliaev Iu A, Marakhova I I, Semenova S B
Tsitologiia. 2008;50(11):953-7.
The recent cloning of the special calcium channels TRPV5 and TRPV6 (transient receptor potential vanilloid channels) provided the molecular base for the studying of new candidate of calcium influx in non-excitable cells. Using RT-PCR technique we obtained endogenous expression of the mRNAs trpv5 and trpv6 in lymphoblast leukemia Jurkat cells and in human blood primary T lymphocytes. Additionally, Western blot analysis showed TRPV5 proteins in both the whole lysate and in the crude membrane preparations from Jurkat cells and normal T lymphocytes. The using of the immunoprecipitation revealed TRPV6 proteins in Jurkat cells, whereas in normal T lymphocytes TRPV6 was not detected. The expression pattern and the selective Ca2+ permeation properties of TRPV5 and TRPV6 channels indicate an important role of these channels in the Ca2+ homeostasis and probably in malignant transformation of blood cells.
近期对特殊钙通道TRPV5和TRPV6（瞬时受体电位香草酸受体通道）的克隆为研究非兴奋性细胞中钙内流的新候选分子提供了分子基础。我们运用逆转录聚合酶链反应（RT-PCR）技术，在淋巴母细胞白血病Jurkat细胞和人血原代T淋巴细胞中获得了trpv5和trpv6 mRNA的内源性表达。此外，蛋白质免疫印迹分析显示，在Jurkat细胞和正常T淋巴细胞的全细胞裂解物及粗制膜制剂中均存在TRPV5蛋白。免疫沉淀法检测发现Jurkat细胞中有TRPV6蛋白，而在正常T淋巴细胞中未检测到TRPV6。TRPV5和TRPV6通道的表达模式及选择性Ca2+通透特性表明，这些通道在Ca2+稳态中以及可能在血细胞的恶性转化过程中发挥着重要作用。
