Tomilin V N, Vasil'eva I O, Marakhova I I, Neguliaev Iu A, Semenova C B
Tsitologiia. 2013;55(5):300-6.
Calcium signaling and Ca(2+)-conducting channels are involved in the development of immune response, cell proliferation, growth and differentiation of lymphocytes. In this paper the calcium channels TRPV5 and TRPV6 (transient receptor potential vanilloid channels) were studied in the plasma membrane of T cell line Jurkat and normal human blood lymphocytes. The channels were activated by removing Ca2+ and Mg2+ from surrounding solution, characterized by inward rectification, and were inactivated by the effective blocker of TRPV5 and TRPV6, ruthenium red. Channel activity was significantly higher in Jurkat cells, than in normal human lymphocytes. Quantitative PCR analysis revealed higher levels of mRNA genes encoding channels TRPV5 and TRPV6 in the proliferation cells compared with resting lymphocytes. In general these data showed that TRPV5/TRPV6 in human lymphocytes are functionally active, and their activity is associated with proliferative status of blood cells.
钙信号传导和钙离子通道参与免疫反应的发展、淋巴细胞的细胞增殖、生长和分化。本文对T细胞系Jurkat和正常人血液淋巴细胞质膜中的钙离子通道TRPV5和TRPV6（瞬时受体电位香草酸受体通道）进行了研究。这些通道通过去除周围溶液中的Ca2+和Mg2+而被激活，具有内向整流特性，并被TRPV5和TRPV6的有效阻滞剂钌红所灭活。Jurkat细胞中的通道活性明显高于正常人淋巴细胞。定量PCR分析显示，与静息淋巴细胞相比，增殖细胞中编码通道TRPV5和TRPV6的mRNA基因水平更高。总体而言，这些数据表明，人淋巴细胞中的TRPV5/TRPV6具有功能活性，其活性与血细胞的增殖状态相关。
