Lin F L, Wu S J, Lee S C, Ng L T
Department of Pharmacy, Tajen University, Pingtung, Taiwan.
Phytother Res. 2009 Jul;23(7):958-64. doi: 10.1002/ptr.2701.
Andrographis paniculata (AP), a popular ingredient of Oriental folk medicine, is commonly used for treating infection, inflammation, fever and diarrhoea. In this study, extracts prepared from cultivated AP and their active constituent andrographolide were evaluated for antioxidant, antioedema and analgesic activities. The results showed that the aqueous AP extract (AP-H2O) exhibited a greater antioxidant activity than the ethanol AP extract (AP-EtOH) in all model systems tested. At a concentration of 50 microg/mL, the free radical scavenging, xanthine oxidase inhibition and antilipid peroxidation activities for AP-H2O were 66.8%, 57.3% and 65.3%, respectively, and for AP-EtOH were 57.8%, 52.6% and 34.2%, respectively. At a dosage of 100 mg/kg, AP-H2O and andrographolide, but not AP-EtOH, showed antioedema and analgesic activities. In phytochemical analysis, AP-H2O showed a higher concentration of total flavanoid but a lower phenol content than AP-EtOH. In conclusion, AP-H2O was more potent than AP-EtOH in antioxidant activities. Furthermore, compared with andrographolide, AP-H2O as an extract also appears to possess potent antioedema and analgesic activities.
穿心莲是东方民间医学中一种常用的成分，常用于治疗感染、炎症、发热和腹泻。在本研究中，对栽培穿心莲制备的提取物及其活性成分穿心莲内酯的抗氧化、抗水肿和镇痛活性进行了评估。结果表明，在所有测试的模型系统中，穿心莲水提取物（AP-H2O）比穿心莲乙醇提取物（AP-EtOH）表现出更强的抗氧化活性。在浓度为50μg/mL时，AP-H2O的自由基清除、黄嘌呤氧化酶抑制和抗脂质过氧化活性分别为66.8%、57.3%和65.3%，而AP-EtOH分别为57.8%、52.6%和34.2%。在剂量为100mg/kg时，AP-H2O和穿心莲内酯表现出抗水肿和镇痛活性，而AP-EtOH则没有。在植物化学分析中，AP-H2O的总黄酮浓度高于AP-EtOH，但酚含量低于AP-EtOH。总之，AP-H2O在抗氧化活性方面比AP-EtOH更有效。此外，与穿心莲内酯相比，AP-H2O作为提取物似乎也具有较强的抗水肿和镇痛活性。
