Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Biol Res Nurs. 2010 Jan;11(3):293-301. doi: 10.1177/1099800409343311. Epub 2009 Aug 18.
The current study was performed to evaluate the antinociceptive and antiedematogenic properties of andrographolide isolated from the leaves of Andrographis paniculata using two animal models. Antinociceptive activity was evaluated using the acetic acid- induced writhing and the hot-plate tests, while antiedematogenic activity was measured using the carrageenan-induced paw edema test. Subcutaneous (s.c.) administration of andrographolide (10, 25, and 50 mg/kg) did not affect the motor coordination of the experimental animals but produced significant (p < .05) antinociceptive activity when assessed using both tests. However, 2 mg/kg naloxone failed to affect the 25 mg/kg andrographolide activity in both tests, indicating that the activity was modulated via nonopioid mechanisms. Furthermore, andrographolide showed significant (p < .05) antiedematogenic activity. In conclusion, the results obtained suggest that andrographolide has antinociceptive and antiedematogenic activities; it may be useful for treating pain and inflammation once human studies are conducted.
本研究旨在评估穿心莲叶中分离得到的穿心莲内酯的镇痛和抗水肿特性，使用了两种动物模型。通过醋酸诱导的扭体和热板试验评估镇痛活性，通过角叉菜胶诱导的足肿胀试验测量抗水肿活性。皮下（s.c.）给予穿心莲内酯（10、25 和 50 mg/kg）不会影响实验动物的运动协调能力，但在两种试验中均产生显著的镇痛活性（p <.05）。然而，2 mg/kg 的纳洛酮未能影响 25 mg/kg 穿心莲内酯在两种试验中的活性，表明该活性是通过非阿片类机制调节的。此外，穿心莲内酯表现出显著的抗水肿活性。总之，研究结果表明，穿心莲内酯具有镇痛和抗水肿活性；在进行人体研究后，它可能对治疗疼痛和炎症有用。
