Jagannathan Jay, Li Jie, Szerlip Nicholas, Vortmeyer Alexander O, Lonser Russell R, Oldfield Edward H, Zhuang Zhengping
Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, NIH, Bethesda, MD 20892-1414, USA.
Neurosurgery. 2009 Jan;64(1):4-14; discussion 14. doi: 10.1227/01.NEU.0000335776.93176.83.
Proteins are the primary components of cells and are vital constituents of any living organism. The proteins that make up an organism (proteome) are constantly changing and are intricately linked to neurological disease processes. The study of proteins, or proteomics, is a relatively new but rapidly expanding field with increasing relevance to neurosurgery.
We present a review of the state-of-the-art proteomic technology and its applications in central nervous system diseases.
The technique of "selective microdissection" allows an investigator to selectively isolate and study a pathological tissue of interest. By evaluating protein expression in a variety of central nervous system disorders, it is clear that proteins are differentially expressed across disease states, and protein expression changes markedly during disease progression.
Understanding the patterns of protein expression in the nervous system has critical implications for the diagnosis and treatment of neurological disease. As gatekeepers in the diagnosis, evaluation, and treatment of central nervous system diseases, it is important for neurosurgeons to develop an appreciation for proteomic techniques and their utility.
蛋白质是细胞的主要成分，是任何生物体的重要组成部分。构成生物体的蛋白质（蛋白质组）不断变化，并且与神经疾病过程有着复杂的联系。蛋白质研究，即蛋白质组学，是一个相对较新但发展迅速的领域，与神经外科的相关性日益增加。
我们对蛋白质组学技术的最新进展及其在中枢神经系统疾病中的应用进行综述。
“选择性显微切割”技术使研究人员能够选择性地分离和研究感兴趣的病理组织。通过评估多种中枢神经系统疾病中的蛋白质表达，很明显蛋白质在不同疾病状态下差异表达，并且在疾病进展过程中蛋白质表达发生显著变化。
了解神经系统中蛋白质表达模式对神经疾病的诊断和治疗具有关键意义。作为中枢神经系统疾病诊断、评估和治疗的把关人，神经外科医生了解蛋白质组学技术及其效用非常重要。
