Rousseau Julia, Barth Rolf F, Moeschberger Melvin L, Elleaume Hélène
Institut National de la Santé et de la Recherche Médicale U836, Equipe 6, Grenoble, France.
Int J Radiat Oncol Biol Phys. 2009 Feb 1;73(2):530-6. doi: 10.1016/j.ijrobp.2008.09.018.
To evaluate the efficacy of prolonged intracerebral (i.c.) administration of carboplatin by means of ALZET osmotic pumps, in combination with radiotherapy for the treatment of intracranial F98 glioma in rats.
Seven days after stereotactic implantation of F98 glioma cells into the brains of Fischer rats, carboplatin was administrated i.c. by means of ALZET pumps over 6 days. Rats were treated at the European Synchrotron Radiation Facility with a single 15-Gy X-ray dose, either given alone or 24 h after administration of carboplatin.
Untreated rats had a mean survival time (MST) +/- SE of 23 +/- 1 days, compared with 44 +/- 3 days for X-irradiated animals and 69 +/- 20 days for rats that received carboplatin alone, with 3 of 13 of these surviving >195 days. Rats that received carboplatin followed by X-irradiation had a MST of >142 +/- 21 days and a median survival time of >195 days, with 6 of 11 rats (55%) still alive at the end of the study. The corresponding percentage increases in lifespan, based on median survival times, were 25%, 85%, and 713%, respectively, for carboplatin alone, radiotherapy alone, or the combination.
Our data demonstrate that i.c. infusion of carboplatin by means of ALZET pumps in combination with X-irradiation is highly effective for the treatment of the F98 glioma. They provide strong support for the approach of concomitantly administering chemo- and radiotherapy for the treatment of brain tumors.
通过ALZET渗透泵对大鼠进行脑内长期注射卡铂，并联合放疗，以评估其对颅内F98胶质瘤的治疗效果。
将F98胶质瘤细胞立体定向植入Fischer大鼠脑内7天后，通过ALZET泵进行为期6天的脑内卡铂注射。大鼠在欧洲同步辐射装置接受单次15 Gy X射线照射，照射可单独进行，也可在卡铂注射后24小时进行。
未治疗的大鼠平均生存时间（MST）±标准误为23±1天，接受X射线照射的动物为44±3天，单独接受卡铂治疗的大鼠为69±20天，其中13只中有3只存活超过195天。先接受卡铂治疗再接受X射线照射的大鼠MST>142±21天，中位生存时间>195天，11只大鼠中有6只（55%）在研究结束时仍存活。基于中位生存时间，单独使用卡铂、单独放疗或联合治疗的寿命相应增加百分比分别为25%、85%和713%。
我们的数据表明，通过ALZET泵脑内输注卡铂联合X射线照射对F98胶质瘤的治疗非常有效。它们为同步进行化疗和放疗治疗脑肿瘤的方法提供了有力支持。
