Ma Luyun, Tu Zeng, Xue Yingwei, Wang Jinfang, Wan Yongji
College of Biotechnology, Southwest University, Chongqing 400716, China.
Wei Sheng Wu Xue Bao. 2008 Nov;48(11):1439-44.
Vairimorpha ceraces is a new microsporidium that isolated from the insect of Lepidoptera, Cerace Stipatana (Walker). We used 16S rDNA sequence to explore genetic status of Vairimorpha ceraces.
SSU rDNA (small subunit ribosomal RNA) of Vairimorpha ceraces was cloned and sequenced for constructing phylogenetic tree through Neighbor-joining.
The core sequence of SSU rRNA of Vairimorpha ceraces with a length of 1228 nucleotides (GenBank EU267796) was successfully cloned and sequenced. Phylogenetic analysis showed that Vairimorpha ceraces was closer to the species of Vairimorpha sp. Germany (GenBank AF124331) and Vairimorpha imperfecta (GenBank AJ131645), both isolated from Plutella xylostella. The three species of genera Vairimorpha included Vairimorpha ceraces formed a clade with the Nosema spp. which from Lepidoptera host in phylogenetic tree. Conversely, other three Vairimorpha spp. from Lepidoptera (Vairimorpha necatrix, Vairimorpha sp. NISM12 and Vairimorpha lymantriae) were mixed with Nosema spp. from non-lepidopteran host in the other clade.
Combined with the biological characters, Vairimorpha ceraces was a member of genera Vairimorpha, Family Nosematidae.
蜡螟微粒子虫是从鳞翅目昆虫角蜡螟（Walker）中分离出的一种新型微孢子虫。我们利用16S rDNA序列来探究蜡螟微粒子虫的遗传状况。
克隆并测序蜡螟微粒子虫的小亚基核糖体RNA（SSU rDNA），通过邻接法构建系统发育树。
成功克隆并测序了长度为1228个核苷酸的蜡螟微粒子虫SSU rRNA核心序列（GenBank登录号EU267796）。系统发育分析表明，蜡螟微粒子虫与同样从小菜蛾中分离出的德国微粒子虫（GenBank登录号AF124331）及不完全微粒子虫（GenBank登录号AJ131645）亲缘关系更近。在系统发育树中，包括蜡螟微粒子虫在内的微粒子虫属的这三个物种与来自鳞翅目宿主的 Nosema 属物种形成一个分支。相反，来自鳞翅目的其他三种微粒子虫（致病微粒子虫、NISM12微粒子虫和舞毒蛾微粒子虫）与来自非鳞翅目宿主的Nosema属物种在另一个分支中混合在一起。
结合生物学特性，蜡螟微粒子虫是微粒子虫属、微粒子科的成员。
