Baker M D, Vossbrinck C R, Maddox J V, Undeen A H
Department of Entomology, University of Illinois, Urbana 61801.
J Invertebr Pathol. 1994 Sep;64(2):100-6. doi: 10.1006/jipa.1994.1077.
A portion (approximately 350 nucleotides) of the large subunit ribosomal RNA (rRNA) 5' to the 580 region (Escherichia coli numbering) was sequenced using the reverse transcriptase dideoxy method and compared for several species of Nosema and Vairimorpha. Comparison among Nosema species suggests that this genus is composed of several unrelated groups. The group which includes the type species, Nosema bombycis, consists of closely related species found primarily in Lepidoptera. Other Nosema species sequenced (Nosema kingi, Nosema algerae, and Nosema locustae) do not appear to be closely related to each other or to the lepidopteran Nosema group. Comparison among the Vairimorpha species indicates that two distinct but very closely related groups exist. The Lymantria group consists of species isolated from the gypsy moth, Lymantria dispar, while the Vairimorpha necatrix group consists of species isolated from other Lepidoptera. Intergeneric comparison of the sequence data suggests that the lepidopteran Nosema species are closely related to the Vairimorpha species.
使用逆转录酶双脱氧法对核糖体大亚基RNA（rRNA）5'端至580区域（大肠杆菌编号）的一部分（约350个核苷酸）进行测序，并对几种微孢子虫属和变形孢虫属的物种进行比较。微孢子虫属物种之间的比较表明，该属由几个不相关的类群组成。包括模式种家蚕微孢子虫在内的类群，主要由在鳞翅目昆虫中发现的亲缘关系密切的物种组成。其他已测序的微孢子虫属物种（金氏微孢子虫、阿尔及利亚微孢子虫和蝗虫微孢子虫）彼此之间以及与鳞翅目微孢子虫类群似乎没有密切关系。变形孢虫属物种之间的比较表明，存在两个不同但亲缘关系非常密切的类群。舞毒蛾类群由从舞毒蛾中分离出的物种组成，而毁灭变形孢虫类群由从其他鳞翅目昆虫中分离出的物种组成。序列数据的属间比较表明，鳞翅目微孢子虫属物种与变形孢虫属物种亲缘关系密切。
