Xu Li-Wei, Shang Ming-Ying, Li Jun, Li Xiao-Mei, Meng Li-Qiang, Cai Shao-Qing
School of Pharmaceutical Science, Peking University, Beijing 100191, China.
Zhongguo Zhong Yao Za Zhi. 2008 Nov;33(21):2508-12.
To analyze and identify the principal composition of ethyl acetate part of Huangqi Danggui decoction by high performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS).
The analysis conditions are as follows: Zorbax SB C18 (4.6 mm x 250 mm, 5 microm) column; mobile phase (A) water mobile phase (B) acetonitrile, gradient elution; UV detection wavelength 254 nm; ESI source and data acquisition in positive and negative mode.
The accurate molecular weights of 20 compounds were measured and identified in ethyl acetate part of Huangqi Danggui decoction. Furthermore, the types of them are as below: flavonoids and flavonoid glycosides, saponins, oligosaccharides, amino acids and phthalides.
It is a rapid and accurate method that the compositions of compound prescription of traditional Chinese medicine can be identified in terms of the separation of high performance liquid chromatography, the accurate molecular weights measured by MS and other information, which can clarify the potential effective compounds of Huangqi Danggui decoction.
采用高效液相色谱 - 电喷雾电离 - 质谱联用（HPLC - ESI - MS）法分析鉴定黄芪当归汤乙酸乙酯部位的主要成分。
分析条件如下：Zorbax SB C18柱（4.6 mm×250 mm，5微米）；流动相（A）为水，流动相（B）为乙腈，梯度洗脱；紫外检测波长254 nm；电喷雾电离源，以正、负离子模式采集数据。
测定并鉴定了黄芪当归汤乙酸乙酯部位中的20种化合物的精确分子量。此外，其类型如下：黄酮类及黄酮苷类、皂苷类、寡糖类、氨基酸类和苯酞类。
利用高效液相色谱的分离、质谱测定的精确分子量等信息鉴定中药复方成分是一种快速、准确的方法，可为阐明黄芪当归汤潜在的有效化合物提供依据。
