Lehti Maarit, Kivelä Riikka, Komi Paavo, Komulainen Jyrki, Kainulainen Heikki, Kyröläinen Heikki
LIKES Research Center, Rautpohjankatu 8, FIN-40700 Jyväskylä, Finland.
J Appl Physiol (1985). 2009 Apr;106(4):1419-24. doi: 10.1152/japplphysiol.90660.2008. Epub 2009 Jan 15.
Eccentric exercise induced by electrostimulation increases mRNA expression of titin-complex proteins in rodent skeletal muscle. In this study, mRNA expression of titin, muscle LIM protein (MLP), cardiac ankyrin repeat protein (CARP), ankyrin repeat domain protein 2 (Ankrd2), diabetes-related ankyrin repeat protein (DARP), and calcium-activated proteinases, calpains, were investigated in human skeletal muscle after fatiguing jumping exercise. Fatiguing jumping exercise did not change mRNA expression of titin, DARP, calpain 1, or calpain 3. MLP, Ankrd2 and calpain 2 mRNA levels were increased 2 days postexercise. CARP mRNA level was already elevated 30 min and remained elevated 2 days postexercise. Increased mRNA expression of MLP, CARP, and Ankrd2, observed for the first time in human skeletal muscle, may be part of the signaling activated by physical exercise. The rapid increase in the level of CARP mRNA nominates CARP as one of the first genes to respond to exercise. The increase in the mRNA level of calpain 2 suggests its involvement in myofiber remodeling after strenuous jumping exercise.
电刺激诱导的离心运动可增加啮齿动物骨骼肌中肌联蛋白复合蛋白的mRNA表达。在本研究中，对疲劳跳跃运动后人骨骼肌中肌联蛋白、肌肉LIM蛋白（MLP）、心肌锚蛋白重复蛋白（CARP）、锚蛋白重复结构域蛋白2（Ankrd2）、糖尿病相关锚蛋白重复蛋白（DARP）以及钙激活蛋白酶（钙蛋白酶）的mRNA表达进行了研究。疲劳跳跃运动并未改变肌联蛋白、DARP、钙蛋白酶1或钙蛋白酶3的mRNA表达。运动后2天，MLP、Ankrd2和钙蛋白酶2的mRNA水平升高。CARP的mRNA水平在运动后30分钟即已升高，并在运动后2天持续升高。在人骨骼肌中首次观察到的MLP、CARP和Ankrd2的mRNA表达增加，可能是体育锻炼激活的信号传导的一部分。CARP mRNA水平的快速升高表明CARP是最早对运动产生反应的基因之一。钙蛋白酶2的mRNA水平升高表明其参与了剧烈跳跃运动后的肌纤维重塑。
