通过上转换实现的超快荧光光谱学在生物物理学中的应用。
Ultrafast fluorescence spectroscopy via upconversion applications to biophysics.
作者信息
Xu Jianhua, Knutson Jay R
机构信息
Laboratory of Molecular Biophysics, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892-1412, USA.
出版信息
Methods Enzymol. 2008;450:159-83. doi: 10.1016/S0076-6879(08)03408-3.
Abstract
This chapter reviews basic concepts of nonlinear fluorescence upconversion, a technique whose temporal resolution is essentially limited only by the pulse width of the ultrafast laser. Design aspects for upconversion spectrophotofluorometers are discussed, and a recently developed system is described. We discuss applications in biophysics, particularly the measurement of time-resolved fluorescence spectra of proteins (with subpicosecond time resolution). Application of this technique to biophysical problems such as dynamics of tryptophan, peptides, proteins, and nucleic acids is reviewed.
摘要
本章回顾了非线性荧光上转换的基本概念，这是一种时间分辨率基本上仅受超快激光脉冲宽度限制的技术。讨论了上转换分光荧光计的设计方面，并描述了一种最近开发的系统。我们讨论了其在生物物理学中的应用，特别是蛋白质时间分辨荧光光谱的测量（具有亚皮秒时间分辨率）。综述了该技术在诸如色氨酸、肽、蛋白质和核酸动力学等生物物理问题上的应用。
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