Gooding L R
J Immunol. 1977 Mar;118(3):920-7.
Cell-mediated immunity to SV40-transformed C3H and C3H-SW cell lines was measured by using both 51Cr and 125IUdR release assays. Killing by cytotoxic cells generated on in vitro sensitization of immune spleen cells with syngeneic SV40 cells by either assay is specific for syngeneic SV40 transformants. Cytolysis mediated by in vitro sensitized cells is ablated by treatment of the effector cells with anti-theta serum and complement. Intraperitoneal immunization with syngeneic SV40 cells yields two distinct killer-cell populations in the peritoneal exudate when assayed by 125IUdR release. The first, nylon wool nonadherent and sensitive to anti-theta and complement, is indistinguishable from the killers generated in vitro. The second population, present in larger numbers and more efficient on a per-cell basis in killing of SV40 targets than the first, is nylon adherent and is not removed by treatment with anti-theta and complement. This second population will kill any SV40 transformed target, whether syngeneic or allogeneic. The possible roles of T cell and non-T cell effectors in rejection of syngeneic SV40 tumors are discussed.
通过使用51Cr和125IUdR释放试验来检测对SV40转化的C3H和C3H-SW细胞系的细胞介导免疫。通过任一试验，用同基因SV40细胞对免疫脾细胞进行体外致敏所产生的细胞毒性细胞的杀伤作用对同基因SV40转化体具有特异性。用抗θ血清和补体处理效应细胞可消除体外致敏细胞介导的细胞溶解作用。当通过125IUdR释放试验检测时，用同基因SV40细胞进行腹腔免疫可在腹腔渗出物中产生两个不同的杀伤细胞群体。第一个群体是尼龙毛不黏附且对抗θ和补体敏感的，与体外产生的杀伤细胞无法区分。第二个群体数量更多，在单个细胞基础上对SV40靶标的杀伤效率比第一个群体更高，它是尼龙黏附的，并且用抗θ和补体处理不能将其去除。第二个群体将杀死任何SV40转化的靶标，无论是同基因的还是异基因的。讨论了T细胞和非T细胞效应器在同基因SV40肿瘤排斥中的可能作用。
