Warnatz H, Krapf F
J Immunol. 1976 Sep;117(3):981-5.
Cytotoxic effector lymphocytes were induced by in vitro immunization of lymph node and spleen cells from AKR-mice (H-2k) and from BALB/c-mice (H-2d) to syngeneic SV40-transformed fibroblasts. The T cell-dependent cytotoxicity was specific for target cells expressing the same H2-specificity as the immunizing cells. Nontransformed fibroblasts as stimulator cells did not induce efficient cytotoxicity to transformed or nontransformed target cells. Incubation with phytohemagglutinin during the sensitization period modified the specificity of the T cell-mediated lysis of syngeneic SV40-transformed fibroblasts: allogeneic as well as syngeneic target cells were destroyed by these effector cells. However, the polyclonal stimulant activates preferentially cytotoxicity to H2-matched target cells. The in vitro generation of cytotoxic effector cells was restricted to living SV40-transformed fibroblasts as immunizing cells; it was not possible to immunize lymphocytes in the presence of membrane proteins prepared from the SV40-transformed cells. The cytotoxicity of the in vitro immunized lymphocytes was inhibited by incubation with membrane protein preparations from syngeneic or allogeneic SV40-transformed fibroblasts.
通过对AKR小鼠（H-2k）和BALB/c小鼠（H-2d）的淋巴结和脾细胞进行体外免疫，使其针对同基因的SV40转化成纤维细胞诱导产生细胞毒性效应淋巴细胞。T细胞依赖性细胞毒性对表达与免疫细胞相同H2特异性的靶细胞具有特异性。未转化的成纤维细胞作为刺激细胞不会对转化或未转化的靶细胞诱导有效的细胞毒性。在致敏期与植物血凝素一起孵育会改变T细胞介导的同基因SV40转化成纤维细胞裂解的特异性：这些效应细胞会破坏同种异体以及同基因靶细胞。然而，多克隆刺激剂优先激活对H2匹配靶细胞的细胞毒性。细胞毒性效应细胞的体外产生仅限于活的SV40转化成纤维细胞作为免疫细胞；在存在由SV40转化细胞制备的膜蛋白的情况下不可能对淋巴细胞进行免疫。体外免疫淋巴细胞的细胞毒性通过与同基因或同种异体SV40转化成纤维细胞的膜蛋白制剂一起孵育而受到抑制。
