Gooding L R, Edwards C B
J Immunol. 1980 Mar;124(3):1258-62.
T cytotoxic cells generated to syngeneic SV40 virus transformants lyse only SV40 target cells that are syngeneic at the H-2 locus. In contrast, SV40-specific tumor transplantation immunity shows no requirements for syngeneic H-2. Inoculation of allogeneic or even xenogeneic transformants will confer immunity to a challenge of syngeneic SV40 tumor cells. The experiments described here represent an attempt to reconcile these apparently conflicting observations. In our hands, generation of SV40-specific T cytotoxic cells in vitro requires both in vivo priming and secondary in vitro sensitization. We have found that priming for a secondary syngeneic-restricted response requires only that the cell employed be SV40 transformed. That is, priming may be accomplished with syngeneic, allogeneic, or xenogeneic SV40 transformants. Thus, the apparent lack of H-2 restriction in vivo immunity does not eliminate a role for the H-2-restricted cytotoxic T cell in tumor transplantation immunity.
针对同基因SV40病毒转化体产生的细胞毒性T细胞仅裂解在H-2位点同基因的SV40靶细胞。相比之下，SV40特异性肿瘤移植免疫对同基因H-2没有要求。接种异基因甚至异种基因转化体将赋予对同基因SV40肿瘤细胞攻击的免疫力。这里描述的实验是试图调和这些明显相互矛盾的观察结果。在我们的实验中，体外产生SV40特异性细胞毒性T细胞需要体内预致敏和二次体外致敏。我们发现，引发二次同基因限制反应仅要求所用细胞是SV40转化的。也就是说，预致敏可以用同基因、异基因或异种基因SV40转化体来完成。因此，体内免疫中明显缺乏H-2限制并不能消除H-2限制的细胞毒性T细胞在肿瘤移植免疫中的作用。
