Lee J C, Ihle J N
J Immunol. 1977 Mar;118(3):928-34.
Spleen cells from normal (B6C3)F1 mice demonstrated natural cytotoxic reactivity mediated by a "null" cell population which, in part, had immunologic specificity for the major envelope glycoprotein (gp71) of the endogenous ecotropic murine leukemia virus. In contrast, the cytotoxic reactivity reflected in spleen cells of NIH Swiss nude mice apparently had no immunologic specificity. A significant level of blastogenic response could be generated in vitro by using normal (B6C3)F1 and AKR spleen cells in the presence of gp71. This reactivity was highly type specific. Moreover, using normal spleen cells from (B6C3)F1, both secondary blastogenic responses and cytotoxic T cell responses could be induced in vitro with purified soluble viral gp71. These findings extend further our previous studies on the existence of a natural immune response in normal mice to their endogenous MuLV by providing in vitro evidence for the expression of cell-mediated response in addition to the humoral response and that at least two effector cell types are operable in the cell-mediated phase.
来自正常（B6C3）F1小鼠的脾细胞表现出由“无标记”细胞群体介导的自然细胞毒性反应，该细胞群体部分对内源性嗜亲性鼠白血病病毒的主要包膜糖蛋白（gp71）具有免疫特异性。相比之下，NIH瑞士裸鼠脾细胞所反映的细胞毒性反应显然没有免疫特异性。在存在gp71的情况下，使用正常（B6C3）F1和AKR脾细胞可在体外产生显著水平的增殖反应。这种反应具有高度的型特异性。此外，使用来自（B6C3）F1的正常脾细胞，纯化的可溶性病毒gp71可在体外诱导二次增殖反应和细胞毒性T细胞反应。这些发现通过提供体外证据，证明除了体液反应外还存在细胞介导反应的表达，并且在细胞介导阶段至少有两种效应细胞类型起作用，进一步扩展了我们之前关于正常小鼠对其内源性MuLV存在自然免疫反应的研究。
