Lee J C, Ihle J N
J Immunol. 1976 Sep;117(3):1033-8.
Xenogeneic and allogeneic antisera to the major envelope glycoprotein (gp71) of murine leukemia viruses (NyLV) inhibited the mitogenic response of normal mouse splenic lymphocytes to phytohemagglutinin (PHA) and lipopolysaccharide (LPS). This inhibition was specific for gp71 as demonstrated by the inability of xenogeneic antisera to other viral glycoproteins or structural proteins to inhibit and by the ability of purified antigens to block specifically the inhibitory effect. The ability of antisera to gp71 to inhibit LPS responses, however, is highly dependent on the strain and age of mouse spleen cells used and appears correlated with the expression of endogenous viruses. Moreover, the preferential inhibition of LPS responses suggests that this expression may be predominately B cell specific. The results suggest that the inhibitory effect is mediated via antibody binding to lymphocytes and that expression of viral envelope antigens on the cell surface which bind immunoglobulins can block or interfere with the binding or uptake of mitogens. A variety of natural mouse immune sera and "tumor" sera, having antibodies directed against gp71, can similarly inhibit mitogen responses; and this inhibition can be specifically blocked with MuLV or gp71.
针对鼠白血病病毒（NyLV）主要包膜糖蛋白（gp71）的异种和同种异体抗血清，抑制了正常小鼠脾淋巴细胞对植物血凝素（PHA）和脂多糖（LPS）的促有丝分裂反应。这种抑制作用对gp71具有特异性，异种抗血清针对其他病毒糖蛋白或结构蛋白时无抑制作用，且纯化抗原能特异性阻断这种抑制作用，均证明了这一点。然而，抗gp71抗血清抑制LPS反应的能力高度依赖于所用小鼠脾细胞的品系和年龄，且似乎与内源性病毒的表达相关。此外，对LPS反应的优先抑制表明这种表达可能主要是B细胞特异性的。结果表明，抑制作用是通过抗体与淋巴细胞结合介导的，细胞表面结合免疫球蛋白的病毒包膜抗原的表达可阻断或干扰有丝分裂原的结合或摄取。多种天然小鼠免疫血清和“肿瘤”血清，因其含有针对gp71的抗体，同样可抑制有丝分裂原反应；且这种抑制作用可用鼠白血病病毒（MuLV）或gp71特异性阻断。
