Vánky F, Klein E
Immunogenetics. 1982 Jan;15(1):31-9. doi: 10.1007/BF00375500.
Interferon activation of lymphocytes produces lytic potential against allogeneic but not against autologous biopsy-derived tumor cells. This effect was seen when targets isolated from solid tumors were used directly without cultivation in vitro. The hypothesis that the lytic effect was due to activation of alloreactive cells was tested in the cold target competition assay. The results substantiated our assumption because they showed that in a given lymphocyte population separate sets act on allogeneic tumor cells derived from different individuals. In addition PHA blasts from the target-cell donor but not from unrelated individuals also inhibited the lysis. The system we use is operationally an NK assay in which antigen (MHC)-recognizing lymphocytes seem to function. Since antigen recognition is a property of the T subset, the conceptual dualism in the classification of NK and CTL effects should be modified.
干扰素激活淋巴细胞可产生针对异体而非自体活检来源肿瘤细胞的裂解潜能。当直接使用从实体瘤分离的靶细胞而不进行体外培养时，就能观察到这种效应。在冷靶竞争试验中对裂解效应是由于同种反应性细胞激活这一假说进行了检验。结果证实了我们的假设，因为结果表明在给定的淋巴细胞群体中，不同的细胞组作用于来自不同个体的异体肿瘤细胞。此外，来自靶细胞供体而非无关个体的PHA母细胞也能抑制裂解。我们所使用的系统在操作上是一种NK检测法，其中识别抗原（MHC）的淋巴细胞似乎发挥作用。由于抗原识别是T亚群的特性，因此NK和CTL效应分类中的概念二元论应予以修正。
