幼鼠皮肤表皮和真皮中前列腺素的生物合成以及钙和环核苷酸的作用。

Prostaglandin biosynthesis in the epidermis and dermis of young mouse skin, and the effects of calcium and cyclic nucleotides.

作者信息

Wilkinson D I, Walsh J T

出版信息

J Invest Dermatol. 1977 Apr;68(4):210-4. doi: 10.1111/1523-1747.ep12493137.

PMID:191537

Abstract

Homogenates of skin from 2- to 3-day old mice converted labeled eicosa-8,11,14-trienoic acid to prostaglandins (PGs) PGE1, D1, and F1alpha. For several reasons PGD2 has been overlooked as a possible major PG in skin. The relative formation of each PG was modified by the presence of reduced glutathione (GSH), chelating agents (EDTA, EGTA), and L-epinephrine. Chelating agents and l-epinephrine increased conversion, espeicially to PGE1 but this effect was not due to inactivation of Ca++ which was shown to have no control over the reaction. Cyclic nucleotides including cyclic AMP (cAMP) and cyclic GMP (cGMP) were also ineffective. The conversion occurred mostly in the epidermis. Epidermal preparations were more efficient in the conversion than those from whole skin. Indomethacin (0.2 mM) completely inhibited the reaction.

摘要

2至3日龄小鼠皮肤匀浆可将标记的8,11,14-二十碳三烯酸转化为前列腺素（PGs）PGE1、D1和F1α。由于多种原因，PGD2作为皮肤中一种可能的主要PG被忽视了。每种PG的相对生成量会因还原型谷胱甘肽（GSH）、螯合剂（EDTA、EGTA）和L-肾上腺素的存在而改变。螯合剂和L-肾上腺素可增加转化率，尤其是对PGE1的转化率，但这种作用并非由于Ca++失活，因为已证明Ca++对该反应无调控作用。包括环磷酸腺苷（cAMP）和环磷酸鸟苷（cGMP）在内的环核苷酸也无效。转化主要发生在表皮。表皮制剂的转化效率高于全皮制剂。吲哚美辛（0.2 mM）可完全抑制该反应。