Chiappa S A, Fink G
J Endocrinol. 1977 Feb;72(2):211-24. doi: 10.1677/joe.0.0720211.
The hypothalamic content of LH releasing factor (RF), pituitary ACTH and pituitary and plasma LH and FSH were measured by radioimmunoassay from foetal Day 15 to postnatal Day 65. Bioassayable corticotrophin releasing activity was also measured during the postnatal period. Hypothalamic LH-RF was detectable as early as foetal Day 15, increasing gradually until postnatal Day 2 and then steeply between Days 5 and 16. The levels of LH-RF were similar in both male and normal female rats until Day 41, after which the increase which had been occurring from Day 16 continued in the male but not the female. In female rats treated with testosterone propionate neonatally ('androgenized females') the hypothalamic content of LH-RF at Day 9 was significantly less than that in the male or normal female, levels reaching those found in the latter two groups by Days 16-22. The lower level of LH-RF in the androgenized female was associated with pituitary gonadotrophin and plasma FSH levels which were lower than in the normal female until Day 30. In the normal female, vaginal opening was associated with a marked drop in hypothalamic LH-RF content and in pituitary LH and FSH, but in the androgenized female, vaginal opening occurred while hypothalamic LH-RF and pituitary LH levels were still rising. The peaks in pituitary FSH and LH and in plasma LH seen on Days 22, 30 and 41, respectively, in the normal female were each delayed by 8-9 days in the androgenized female. In all three types of animal there was a significant drop in plasma FSH between Days 50 and 65 which was associated with a significant increase in pituitary FSH in the male and a significant decrease in pituitary FSH in the androgenized female rats. The day 17 foetal pituitary gland also contained ACTH, and again levels of this hormone rose steeply between Days 5 and 9. In contrast to the gonadotrophins, there was a marked divergence between the pituitary content and concentration of ACTH: content rose while concentration remained relatively steady after Day 9. There was no major difference in pituitary ACTH levels between the three types of animal throughout the study; however, around Days 16 and 50, corticotrophin releasing activity was higher in males and androgenized females compared with that in normal females.
采用放射免疫分析法测定了从胚胎第15天到出生后第65天下丘脑促黄体生成素释放因子（RF）、垂体促肾上腺皮质激素（ACTH）以及垂体和血浆中促黄体生成素（LH）和促卵泡激素（FSH）的含量。在出生后阶段还测定了具有生物活性的促肾上腺皮质激素释放活性。下丘脑促黄体生成素释放因子早在胚胎第15天即可检测到，逐渐增加直至出生后第2天，然后在第5天至第16天急剧上升。直到第41天，雄性和正常雌性大鼠的促黄体生成素释放因子水平相似，此后，从第16天开始的增加在雄性大鼠中持续，但在雌性大鼠中不再继续。在新生期用丙酸睾酮处理的雌性大鼠（“雄激素化雌性大鼠”）中，第9天促黄体生成素释放因子的下丘脑含量显著低于雄性或正常雌性大鼠，到第16 - 22天达到后两组的水平。雄激素化雌性大鼠促黄体生成素释放因子水平较低，与之相关的是垂体促性腺激素和血浆促卵泡激素水平，直到第30天均低于正常雌性大鼠。在正常雌性大鼠中，阴道开口与下丘脑促黄体生成素释放因子含量以及垂体促黄体生成素和促卵泡激素的显著下降有关，但在雄激素化雌性大鼠中，阴道开口时下丘脑促黄体生成素释放因子和垂体促黄体生成素水平仍在上升。正常雌性大鼠分别在第22天、30天和41天出现的垂体促卵泡激素和促黄体生成素以及血浆促黄体生成素峰值，在雄激素化雌性大鼠中均延迟8 - 9天出现。在所有三种类型的动物中，第50天至第65天血浆促卵泡激素均显著下降，这与雄性大鼠垂体促卵泡激素显著增加以及雄激素化雌性大鼠垂体促卵泡激素显著减少有关。胚胎第17天的垂体也含有促肾上腺皮质激素，该激素水平同样在第5天至第9天急剧上升。与促性腺激素不同的是，垂体促肾上腺皮质激素的含量和浓度存在明显差异：含量上升，而浓度在第9天后保持相对稳定。在整个研究过程中，三种类型动物的垂体促肾上腺皮质激素水平没有重大差异；然而，在第16天和第50天左右，雄性和雄激素化雌性大鼠的促肾上腺皮质激素释放活性高于正常雌性大鼠。
