Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.
Glycoconj J. 2009 Nov;26(8):1055-64. doi: 10.1007/s10719-008-9226-5.
MALDI-TOF mass spectrometry, negative ion nano-electrospray MS/MS and exoglycosidase digestion were used to identify 36 N-linked glycans from 19S IgM heavy chain derived from the nurse shark (Ginglymostoma cirratum). The major glycan was the high-mannose compound, Man(6)GlcNAc(2) accompanied by small amounts of Man(5)GlcNAc(2), Man(7)GlcNAc(2) and Man(8)GlcNAc(2). Bi- and tri-antennary (isomer with a branched 3-antenna) complex-type glycans were also abundant, most contained a bisecting GlcNAc residue (beta1-->4-linked to the central mannose) and with varying numbers of alpha-galactose residues capping the antennae. Small amounts of monosialylated glycans were also found. This appears to be the first comprehensive study of glycosylation in this species of animal. The glycosylation pattern has implications for the mechanism of activation of the complement system by nurse shark IgM.
基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）、负离子纳米电喷雾串联质谱（negative ion nano-electrospray MS/MS）和外糖苷酶消化法用于鉴定从护士鲨（Ginglymostoma cirratum）19S IgM 重链衍生的 36 个 N-连接聚糖。主要的糖基化产物是高甘露糖化合物 Man(6)GlcNAc(2)，还含有少量的 Man(5)GlcNAc(2)、Man(7)GlcNAc(2)和 Man(8)GlcNAc(2)。双触角和三触角（具有分支 3 触角的异构体）复合型糖基化也很丰富，大多数含有一个双连接的 GlcNAc 残基（β1-->4 连接到中央甘露糖），并且在触角上有不同数量的α-半乳糖残基。还发现了少量的单唾液酸化聚糖。这似乎是该动物物种糖基化的首次全面研究。这种糖基化模式对护士鲨 IgM 激活补体系统的机制具有重要意义。
Zotero 插件