Magnadóttir Bergljót, Crispin Max, Royle Louise, Colominas Cristina, Harvey David J, Dwek Raymond A, Rudd Pauline M
Institute for Experimental Pathology, University of Iceland, Reykjavík, Iceland.
Fish Shellfish Immunol. 2002 Mar;12(3):209-27. doi: 10.1006/fsim.2001.0364.
The carbohydrate moiety of cod serum IgM was analysed using oligosaccharide sequencing techniques. The carbohydrate moiety constituted about 10% of the molecular weight of cod IgM, was associated with the constant region of the heavy chains (Fc), and was composed of N-linked complex type oligosaccharides. Considerable heterogeneity was observed. Sixteen different glycan structures were identified, over 60% were sialylated and 40% contained core fucose. The carbohydrate moiety of cod IgM was shown to provide protection against protease digestion, and partial deglycosylation abolished the antigen binding property of natural cod anti-TNP-BSA antibody.
