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本文引用的文献

1
N-Glycan structures of squid rhodopsin.鱿鱼视紫红质的N-聚糖结构
Eur J Biochem. 2003 Jun;270(12):2627-32. doi: 10.1046/j.1432-1033.2003.03636.x.
2
Schistosome N-glycans containing core alpha 3-fucose and core beta 2-xylose epitopes are strong inducers of Th2 responses in mice.含有核心α3-岩藻糖和核心β2-木糖表位的血吸虫N-聚糖是小鼠Th2反应的强诱导剂。
Eur J Immunol. 2003 May;33(5):1271-81. doi: 10.1002/eji.200323717.
3
A phase I study of autologous dendritic cell-based immunotherapy for patients with unresectable primary liver cancer.一项针对不可切除原发性肝癌患者的基于自体树突状细胞免疫疗法的I期研究。
Cancer Immunol Immunother. 2003 Mar;52(3):155-61. doi: 10.1007/s00262-002-0360-9. Epub 2003 Feb 6.
4
Structural determination of the N-glycans of a lepidopteran arylphorin reveals the presence of a monoglucosylated oligosaccharide in the storage protein.一种鳞翅目芳基载体蛋白N-聚糖的结构测定揭示了该储存蛋白中存在单葡萄糖基化寡糖。
Glycobiology. 2003 Mar;13(3):147-57. doi: 10.1093/glycob/cwg023. Epub 2002 Dec 17.
5
Dendritic cell KLH loading requirements for efficient CD4+ T-cell priming and help to peptide-specific cytotoxic T-cell response, in view of potential use in cancer vaccines.鉴于在癌症疫苗中的潜在应用，探讨树突状细胞负载钥孔戚血蓝蛋白以有效启动CD4+ T细胞并辅助针对肽特异性细胞毒性T细胞反应的负载要求。
Vaccine. 2003 Feb 14;21(9-10):869-76. doi: 10.1016/s0264-410x(02)00534-0.
6
In vitro anticancer effects of a novel immunostimulant: keyhole limpet hemocyanin.一种新型免疫刺激剂：匙孔血蓝蛋白的体外抗癌作用
J Surg Res. 2002 Dec;108(2):279-84. doi: 10.1006/jsre.2002.6548.
7
Hemocyanin from the keyhole limpet Megathura crenulata (KLH) carries a novel type of N-glycans with Gal(beta1-6)Man-motifs.来自钥孔帽贝（Megathura crenulata，KLH）的血蓝蛋白携带一种新型的带有Gal(β1-6)Man基序的N-聚糖。
Eur J Biochem. 2002 Nov;269(22):5459-73. doi: 10.1046/j.1432-1033.2002.03244.x.
8
Antigenicity and immunogenicity of phage library-selected peptide mimics of the major surface proteophosphoglycan antigens of Entamoeba histolytica.溶组织内阿米巴主要表面蛋白磷酸聚糖抗原的噬菌体文库筛选肽模拟物的抗原性和免疫原性。
Parasite Immunol. 2002 Jun;24(6):321-8. doi: 10.1046/j.1365-3024.2002.00463.x.
9
Immunotherapy for bladder cancer.膀胱癌的免疫疗法。
Curr Urol Rep. 2001 Feb;2(1):62-9. doi: 10.1007/s11934-001-0027-7.
10
Generation and characterization of antibodies to sulfated glucuronyl glycolipids in Lewis rats.Lewis大鼠中硫酸化葡糖醛酸糖脂抗体的产生及特性分析
J Neuroimmunol. 2002 Jun;127(1-2):54-8. doi: 10.1016/s0165-5728(02)00094-2.

一种新型的Gal(β1-4)Gal(β1-4)Fuc(α1-6)核心修饰连接在钥孔戚血蓝蛋白（KLH）N-聚糖近端的N-乙酰葡糖胺上。

A novel Gal(beta1-4)Gal(beta1-4)Fuc(alpha1-6)-core modification attached to the proximal N-acetylglucosamine of keyhole limpet haemocyanin (KLH) N-glycans.

作者信息

Wuhrer Manfred, Robijn Marjolein L M, Koeleman Carolien A M, Balog Crina I A, Geyer Rudolf, Deelder André M, Hokke Cornelis H

机构信息

Department of Parasitology, Center of Infectious Diseases, Leiden University Medical Center, P.O. Box 9600, 2300 RC Leiden, The Netherlands.

出版信息

Biochem J. 2004 Mar 1;378(Pt 2):625-32. doi: 10.1042/BJ20031380.

DOI:10.1042/BJ20031380
PMID:14613482
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1223963/
Abstract

KLH (keyhole limpet haemocyanin), the oxygen-carrying molecule of the marine snail Megathura crenulata, is often used as an adjuvant or as a hapten carrier for immunizations with peptides, oligosaccharides or other low-molecular-mass organic compounds. KLH exhibits several carbohydrate determinants, at least some of which are immunogenic: it shares an antigenic Fuc(alpha1-3)GalNAc-determinant with schistosomes and contains unique Gal-(beta1-6)Man-structural motifs on its N-glycans. This study reveals the presence of N-glycans with unusual +/-Gal(beta1-4)Gal(beta1-4)Fuc- units (alpha1-6)-linked to the reducing end N -acetylglucosamine residue. The following novel structures of KLH N-glycans were deduced by linkage analysis, exoglycosidase digestion, matrix-assisted laser-desorption ionization-tandem MS and nano-LC-ESI-IT-MS (where LC stands for liquid chromatography, ESI for electrospray ionization and IT for ion trap): Man(alpha1-6)[+/-Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)[Gal(beta1-4)Fuc(alpha1-6)]GlcNAc and Man(alpha1-6)Man(beta1-4)GlcNAc(beta1-4)[Gal(beta1-4)Gal(beta1-4)Fuc(alpha1-6)]GlcNAc. The Gal(beta1-4)Fuc- and Gal(beta1-4)Gal(beta1-4)Fuc- core modifications are expected to be immunogenic, similar to other non-mammalian-type core modifications, and to contribute to the immunostimulatory properties of KLH.

摘要

钥孔血蓝蛋白（KLH）是海蜗牛巨缘螺的携氧分子，常被用作佐剂或作为肽、寡糖或其他低分子量有机化合物免疫接种的半抗原载体。KLH具有多种碳水化合物决定簇，其中至少一些具有免疫原性：它与血吸虫共享一个抗原性的Fuc（α1-3）GalNAc决定簇，并且在其N-聚糖上含有独特的Gal-（β1-6）Man结构基序。本研究揭示了存在与还原端N-乙酰葡糖胺残基以（α1-6）连接的具有不寻常的+/-Gal（β1-4）Gal（β1-4）Fuc-单元的N-聚糖。通过连接分析推导了KLH N-聚糖的以下新结构，外切糖苷酶消化、基质辅助激光解吸电离串联质谱和纳米液相色谱-电喷雾电离-离子阱质谱（其中LC代表液相色谱，ESI代表电喷雾电离，IT代表离子阱）：Man（α1-6）[+/-Man（α1-3）]Man（β1-4）GlcNAc（β1-4）[Gal（β1-4）Fuc（α1-6）]GlcNAc和Man（α1-6）Man（β1-4）GlcNAc（β1-4）[Gal（β1-4）Gal（β1-4）Fuc（α1-6）]GlcNAc。预计Gal（β1-4）Fuc-和Gal（β1-4）Gal（β1-4）Fuc-核心修饰与其他非哺乳动物型核心修饰类似，具有免疫原性，并有助于KLH的免疫刺激特性。