一种新型的Gal(β1-4)Gal(β1-4)Fuc(α1-6)核心修饰连接在钥孔戚血蓝蛋白（KLH）N-聚糖近端的N-乙酰葡糖胺上。
A novel Gal(beta1-4)Gal(beta1-4)Fuc(alpha1-6)-core modification attached to the proximal N-acetylglucosamine of keyhole limpet haemocyanin (KLH) N-glycans.
作者信息
Wuhrer Manfred, Robijn Marjolein L M, Koeleman Carolien A M, Balog Crina I A, Geyer Rudolf, Deelder André M, Hokke Cornelis H
机构信息
Department of Parasitology, Center of Infectious Diseases, Leiden University Medical Center, P.O. Box 9600, 2300 RC Leiden, The Netherlands.
出版信息
Biochem J. 2004 Mar 1;378(Pt 2):625-32. doi: 10.1042/BJ20031380.
KLH (keyhole limpet haemocyanin), the oxygen-carrying molecule of the marine snail Megathura crenulata, is often used as an adjuvant or as a hapten carrier for immunizations with peptides, oligosaccharides or other low-molecular-mass organic compounds. KLH exhibits several carbohydrate determinants, at least some of which are immunogenic: it shares an antigenic Fuc(alpha1-3)GalNAc-determinant with schistosomes and contains unique Gal-(beta1-6)Man-structural motifs on its N-glycans. This study reveals the presence of N-glycans with unusual +/-Gal(beta1-4)Gal(beta1-4)Fuc- units (alpha1-6)-linked to the reducing end N -acetylglucosamine residue. The following novel structures of KLH N-glycans were deduced by linkage analysis, exoglycosidase digestion, matrix-assisted laser-desorption ionization-tandem MS and nano-LC-ESI-IT-MS (where LC stands for liquid chromatography, ESI for electrospray ionization and IT for ion trap): Man(alpha1-6)[+/-Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)[Gal(beta1-4)Fuc(alpha1-6)]GlcNAc and Man(alpha1-6)Man(beta1-4)GlcNAc(beta1-4)[Gal(beta1-4)Gal(beta1-4)Fuc(alpha1-6)]GlcNAc. The Gal(beta1-4)Fuc- and Gal(beta1-4)Gal(beta1-4)Fuc- core modifications are expected to be immunogenic, similar to other non-mammalian-type core modifications, and to contribute to the immunostimulatory properties of KLH.
钥孔血蓝蛋白（KLH）是海蜗牛巨缘螺的携氧分子，常被用作佐剂或作为肽、寡糖或其他低分子量有机化合物免疫接种的半抗原载体。KLH具有多种碳水化合物决定簇，其中至少一些具有免疫原性：它与血吸虫共享一个抗原性的Fuc（α1-3）GalNAc决定簇，并且在其N-聚糖上含有独特的Gal-（β1-6）Man结构基序。本研究揭示了存在与还原端N-乙酰葡糖胺残基以（α1-6）连接的具有不寻常的+/-Gal（β1-4）Gal（β1-4）Fuc-单元的N-聚糖。通过连接分析推导了KLH N-聚糖的以下新结构，外切糖苷酶消化、基质辅助激光解吸电离串联质谱和纳米液相色谱-电喷雾电离-离子阱质谱（其中LC代表液相色谱，ESI代表电喷雾电离，IT代表离子阱）：Man（α1-6）[+/-Man（α1-3）]Man（β1-4）GlcNAc（β1-4）[Gal（β1-4）Fuc（α1-6）]GlcNAc和Man（α1-6）Man（β1-4）GlcNAc（β1-4）[Gal（β1-4）Gal（β1-4）Fuc（α1-6）]GlcNAc。预计Gal（β1-4）Fuc-和Gal（β1-4）Gal（β1-4）Fuc-核心修饰与其他非哺乳动物型核心修饰类似，具有免疫原性，并有助于KLH的免疫刺激特性。
Zotero 插件