用于蛋白质点击化学的吡咯赖氨酸类似物。
A pyrrolysine analogue for protein click chemistry.
作者信息
Fekner Tomasz, Li Xin, Lee Marianne M, Chan Michael K
机构信息
Department of Chemistry, The Ohio State University, 484 West 12th Avenue, Columbus, OH 43210, USA.
出版信息
Angew Chem Int Ed Engl. 2009;48(9):1633-5. doi: 10.1002/anie.200805420.
Ignoring the STOP sign: A pyrrolysine analogue bearing a terminal alkyne was site-specifically incorporated into recombinant calmodulin (CaM) through a UAG codon. The resulting protein was labeled with an azide-containing dye using a copper(I)-catalyzed click reaction. Subsequent application of an orthogonal cysteine tagging method yielded a CaM labeled with two distinct fluorophores that enabled its study by FRET spectroscopy.
无视终止信号
一种带有末端炔烃的吡咯赖氨酸类似物通过UAG密码子位点特异性地掺入重组钙调蛋白（CaM）中。使用铜（I）催化的点击反应，用含叠氮化物的染料对所得蛋白质进行标记。随后应用正交半胱氨酸标记方法得到了用两种不同荧光团标记的CaM，这使得能够通过荧光共振能量转移（FRET）光谱对其进行研究。
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