[不同产地枳壳的毛细管电泳指纹图谱研究]

[Study on fingerprints of Citrus aurantium from different places by capillary electrophoresis].

作者信息

Luo Guang-Ming, Tan Xiao-Hui, Xu Li-Fang, Yang Ya-Qin, Yang Shi-Lin

机构信息

Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2008 Oct;33(20):2362-4.

PMID:19157129

Abstract

OBJECTIVE

To develop a high performance capillary electrophoresis method for Citrus aurantium fingerprints to control its quality.

METHOD

The background electrolyte (BGE) was an 80 mmol x L(-1) boric acid solution containing 15 mmol x L(-1) borate. The pH of the BGE was adjusted to 9.70 with KOH solution. The detection wavelength was 201 nm and a voltage of 16 kV was applied. The sample hydrodynamic injection was 0.4 ps with a duration time of 8 sec. C. aurantium was extracted by water and a set of capillary electrophoresis fingerprints (CEFP) containing 12 co-possessing peaks was obtained.

RESULT

There were good similarities between the standard CEFP and each set of CEFP of C. aurantium collected from eleven different places, and their similarity coefficients were between 0.973 and 0.996.

CONCLUSION

The CEFP has acceptable precision, reproducibility and stability and can be used for the quality control of C. aurantium.

摘要

目的

建立一种用于枳壳指纹图谱分析以控制其质量的高效毛细管电泳方法。

方法

背景电解质（BGE）为含15 mmol·L⁻¹硼酸盐的80 mmol·L⁻¹硼酸溶液，用氢氧化钾溶液将BGE的pH值调至9.70。检测波长为201 nm，施加电压16 kV。样品采用流体动力学进样，进样量0.4 psi，持续时间8秒。枳壳用水提取，获得了一组包含12个共有峰的毛细管电泳指纹图谱（CEFP）。