[广西光润金线蛭高效毛细管电泳指纹图谱研究]

[Study on HPCE fingerprints of Poecilobdella manillensis from Guangxi province].

作者信息

Jiang Xingang, Meng Zuohuan, Jia Jiming

机构信息

Shijiazhuang Yiling Pharmaceutical Co., Ltd., Shijiazhuang 050035, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2012 Feb;37(4):495-9.

PMID:22667151

Abstract

OBJECTIVE

To establish fingerprints for Poecilobdella manillensis from Guangxi province using the high performance capillary electrophoresis (HPCE) method.

METHOD

Electrophoresis was performed on a fused silica capillary column (75 microm x 56 cm), with 25 mmol x L(-1) Na2HPO4-120 mmol x L(-1) Tris-16 mmol x L(-1) SDS (adjusted to pH 12.0 with 1 mol x L(-1) NaOH ) as the running buffer. The applied voltage was 17 kV, the temperature was 25 degrees C and the detection wavelength was 254 nm. The sample's hydrodynamic injection was 3.4 kPa x 6s and the duration was 27 min.

RESULT

HPCE fingerprint was established with 13 common peaks. The similarity between fingerprints of P. manillensis in 10 batches and control fingerprints was more than 0.98.

CONCLUSION

The method is so precise, reproducible and stable that it could be used as a new means for the quality control of P. manillensis.

摘要

目的

采用高效毛细管电泳（HPCE）法建立广西马尼拉宽体金线蛭的指纹图谱。

方法

在熔融石英毛细管柱（75μm×56cm）上进行电泳，以25mmol·L⁻¹ Na₂HPO₄ - 120mmol·L⁻¹ Tris - 16mmol·L⁻¹ SDS（用1mol·L⁻¹ NaOH调至pH 12.0）作为运行缓冲液。施加电压为17kV，温度为25℃，检测波长为254nm。样品的流体动力学进样为3.4kPa×6s，持续时间为27min。