Payne T, Newmark J, Reid K H
Department of Neurology, School of Medicine, University of Louisville, Kentucky.
Exp Neurol. 1991 Oct;114(1):66-72. doi: 10.1016/0014-4886(91)90085-q.
We have produced controlled local demyelination in Wistar rat fimbriae by injection of microliter quantities of the detergent lysophosphatidyl choline (LPC) stereotactically. Six to seven days later the hippocampus and fornix was dissected out en bloc and maintained in vitro for electrical evaluation of conduction through the damaged area. The tissue was then fixed for verification of the lesion by light and electron microscopy. All fimbriae showed a normal conducted action potential with a latency of 0.5 to 1.5 ms when the conduction distance was 4-5 mm. LPC-lesioned fimbriae also showed a later wave with a latency of 4-5 ms. This later wave had the same stimulus-response curve as the primary action potential, but was more sensitive to repeated stimulation. We interpret this wave as evidence of conduction into or through a demyelinated region. LPC-lesioned fimbriae also showed histological evidence of demyelination. This preparation provides an in vitro model for the study of acute local demyelination of central nervous system white matter, such as that induced by multiple sclerosis and other focally demyelinating diseases.
我们通过立体定位注射微升量的去污剂溶血磷脂酰胆碱（LPC），在Wistar大鼠的海马伞中制造了可控的局部脱髓鞘。六到七天后，将海马体和穹窿整体取出，在体外进行维持，以对通过受损区域的传导进行电学评估。然后将组织固定，通过光学显微镜和电子显微镜来验证损伤情况。当传导距离为4 - 5毫米时，所有正常的海马伞均显示出潜伏期为0.5至1.5毫秒的正常动作电位。经LPC损伤的海马伞还显示出潜伏期为4 - 5毫秒的后续波。这个后续波与初级动作电位具有相同的刺激 - 反应曲线，但对重复刺激更为敏感。我们将此波解释为传导进入或通过脱髓鞘区域的证据。经LPC损伤的海马伞还显示出脱髓鞘的组织学证据。该制备方法为研究中枢神经系统白质的急性局部脱髓鞘提供了一个体外模型，例如由多发性硬化症和其他局灶性脱髓鞘疾病所诱发的脱髓鞘。
