Waxman S G, Kocsis J D, Nitta K C
J Neurol Sci. 1979 Dec;44(1):45-53. doi: 10.1016/0022-510x(79)90221-1.
The local application of lysophosphatidyl choline (LPC) by microinjection into the region of the corpus callosum of the rabbit produced demyelinating lesions. The lesions were assessed histologically using the Luxol fast blue myelin stain and the Holmes silver nitrate stain for the axis cylinders. Survival times for the animals ranged from 7 to 14 days. The center of the lesion was marked by infiltration of macrophages and necrosis, but the major area of the lesion was characterized by demyelination. By consideration of anatomical factors influencing LPC diffusion and of the appropriate placement of the injection, the entire vertical extent (about 0.5 mm) of the corpus callosum could be demyelinated with minimal amounts of necrosis. Since focal demyelination was possible in the fine caliber axons of the corpus callosum which are anatomically representative of many forebrain fiber systems, and since this fiber system is amenable to chronic physiological investigation, the corpus callosum may serve as an experimental model for morpho-physiological studies of mammalian central demyelinating pathways.
通过微量注射将溶血磷脂酰胆碱（LPC）局部应用于兔胼胝体区域可产生脱髓鞘病变。使用Luxol固蓝髓鞘染色法和用于轴突圆柱体的福尔摩斯硝酸银染色法对病变进行组织学评估。动物的存活时间为7至14天。病变中心以巨噬细胞浸润和坏死为特征，但病变的主要区域以脱髓鞘为特征。通过考虑影响LPC扩散的解剖学因素以及注射的适当位置，可以用最少的坏死量使胼胝体的整个垂直范围（约0.5毫米）脱髓鞘。由于在胼胝体的细口径轴突中可能发生局灶性脱髓鞘，这些轴突在解剖学上代表许多前脑纤维系统，并且由于该纤维系统适合进行慢性生理学研究，因此胼胝体可作为哺乳动物中枢脱髓鞘途径形态生理学研究的实验模型。
