左旋肉碱对钙超载大鼠肝线粒体的保护作用。

The protecting effect of L-carnitine on Ca(2+)-loaded rat liver mitochondria.

作者信息

Mokhova E, Arrigoni-Martelli E, Bellei M, Dedukhova V, Muscatello U, Starkov A, Bobyleva V

机构信息

Institute of General Pathology, Modena, Italy.

出版信息

FEBS Lett. 1991 Sep 9;289(2):187-9. doi: 10.1016/0014-5793(91)81066-h.

DOI:10.1016/0014-5793(91)81066-h

PMID:1915847

Abstract

It is shown that L-carnitine strongly increases the ability of rat liver mitochondria to respond to the train of Ca2+ additions by a transient stimulation of the State-4 respiration rate. Such an effect requires ATP and the L-carnitine efficiency strongly decreases when ATP is omitted. Oleate influences the mitochondria in a fashion opposite to that of L-carnitine. The oleate effect is strongly diminished by L-carnitine. Again, the L-carnitine effect requires ATP, and D-carnitine fails to substitute for L-carnitine. It is suggested that L-carnitine removes, in an ATP-dependent manner, endogenous or added fatty acids, which are involved in oxidative damage of Ca(2+)-loaded mitochondria.

摘要

结果表明，左旋肉碱能显著增强大鼠肝脏线粒体对一系列钙离子添加的反应能力，通过短暂刺激状态4呼吸速率来实现。这种效应需要ATP，当省略ATP时，左旋肉碱的效率会大幅降低。油酸对线粒体的影响与左旋肉碱相反。左旋肉碱能显著减弱油酸的作用。同样，左旋肉碱的作用需要ATP，而右旋肉碱不能替代左旋肉碱。研究表明，左旋肉碱以ATP依赖的方式清除内源性或添加的脂肪酸，这些脂肪酸参与了钙离子负载线粒体的氧化损伤。