Bisby R H, Parker A W
Department of Biological Sciences, University of Salford, UK.
FEBS Lett. 1991 Sep 23;290(1-2):205-8. doi: 10.1016/0014-5793(91)81260-f.
Laser flash photolysis of alpha-tocopherol in methanol and in aqueous micellar solutions has been shown to produce the alpha-tocopheroxyl radical. The reaction between the alpha-tocopheroxyl radical and ascorbate in positively charged hexadecyltrimethylammonium chloride (HTAC) micelles occurred with a second order rate constant of 7.2 x 10(7) M-1.s-1, whereas in negatively charged sodium dodecyl sulphate (SDS) micelles the rats constant was only 3.8 x 10(4) M-1.s-1. The alpha-tocopheroxyl radical was found to be relatively long-lived in HTAC micelles (t1/2 greater than or equal to 5 min), allowing the slow disappearance of the alpha-tocopheroxyl radical by reaction with glutathione to be observed.
已证明，在甲醇和胶束水溶液中，α-生育酚经激光闪光光解会产生α-生育酚氧自由基。在带正电荷的十六烷基三甲基氯化铵（HTAC）胶束中，α-生育酚氧自由基与抗坏血酸盐之间反应的二级速率常数为7.2×10⁷ M⁻¹·s⁻¹，而在带负电荷的十二烷基硫酸钠（SDS）胶束中，该速率常数仅为3.8×10⁴ M⁻¹·s⁻¹。研究发现，α-生育酚氧自由基在HTAC胶束中的寿命相对较长（半衰期大于或等于5分钟），从而能够观察到其与谷胱甘肽反应导致的缓慢消失过程。
