Mortensen A, Skibsted L H
Department of Dairy and Food Science, Royal Veterinary and Agricultural University, Frederiksberg C, Denmark.
FEBS Lett. 1997 Nov 17;417(3):261-6. doi: 10.1016/s0014-5793(97)01297-0.
Real time detection following laser flash photolysis of transient carotenoid radical cations and tocopheroxyl radicals formed in chloroform and bleaching of the carotenoids has allowed interaction between carotenoids and tocopherols to be studied. It is found that alpha-, beta-, and gamma-tocopherol reduce all the carotenoid radical cations investigated whereas the delta-tocopheroxyl radical can be reduced by lycopene and beta-carotene. Astaxanthin, canthaxanthin, and beta-apo-8'-carotenal radical cations are scavenged rapidly by all four tocopherol homologues whereas the other carotenoid radical cations react much more slowly with the tocopherols. The results allow the antioxidant hierarchy to be established: alpha-tocopherol > lycopene approximately beta-tocopherol approximately gamma-tocopherol > beta-carotene > zeaxanthin approximately delta-tocopherol > lutein > echinenone >> canthaxanthin approximately beta-apo-8'-carotenal > astaxanthin.
通过激光闪光光解氯仿中形成的瞬态类胡萝卜素自由基阳离子和生育酚自由基以及类胡萝卜素的漂白进行实时检测，使得对类胡萝卜素与生育酚之间的相互作用进行研究成为可能。研究发现，α-、β-和γ-生育酚能还原所有所研究的类胡萝卜素自由基阳离子，而δ-生育酚自由基可被番茄红素和β-胡萝卜素还原。虾青素、角黄素和β-阿朴-8'-胡萝卜醛自由基阳离子能被所有四种生育酚同系物迅速清除，而其他类胡萝卜素自由基阳离子与生育酚的反应则慢得多。这些结果使得抗氧化剂层级得以确立：α-生育酚＞番茄红素≈β-生育酚≈γ-生育酚＞β-胡萝卜素＞玉米黄质≈δ-生育酚＞叶黄素＞海胆酮＞＞角黄素≈β-阿朴-8'-胡萝卜醛＞虾青素。
