Castillo I, Bartolomé J, Madejón A, Melero M, Porres J C, Carreño V
Department of Gastroenterology, Fundación Jiménez Díaz, Madrid, Spain.
Digestion. 1991;48(3):149-56. doi: 10.1159/000200687.
Hepatitis delta virus (HDV) RNA detection was carried out, using a full-length HDV RNA probe, in serum of 43 patients with chronic HDV infection. Among them, 30 cases (70%) were HDV RNA-positive. With respect to other HDV markers, serum HDAg (detected by immunoblot) was found in 33 patients (77%) and IgM anti-HD in 29 (67%). A similar percentage of HDV RNA-positive patients with and without circulating hepatitis B virus (HBV) DNA (32.5 vs. 37%, respectively) was found. Antibodies against the human immunodeficiency virus (HIV) were detected in 15/43 subjects studied. The presence of HDV RNA was significantly higher (p less than 0.05) in anti-HIV-seropositive cases (93%) than in the HIV-seronegative ones (57%). Moreover, simultaneous HDV and HBV replication was found more frequently (60 vs. 18%, p less than 0.05) and at higher levels among the anti-HIV-positive patients than in the rest. In addition, in most of the anti-HIV-positive subjects, HDV RNA and HBV DNA were constantly positive during a whole year of follow-up.
采用全长丁型肝炎病毒（HDV）RNA探针，对43例慢性HDV感染患者的血清进行HDV RNA检测。其中，30例（70%）HDV RNA呈阳性。关于其他HDV标志物，33例患者（77%）血清中检测到HDAg（通过免疫印迹法检测），29例（67%）血清中检测到IgM抗-HD。在HDV RNA阳性患者中，检测到和未检测到循环乙型肝炎病毒（HBV）DNA的患者比例相似（分别为32.5%和37%）。在43例研究对象中，15例检测到抗人类免疫缺陷病毒（HIV）抗体。抗HIV血清学阳性病例中HDV RNA的存在率（93%）显著高于HIV血清学阴性病例（57%）（p<0.05）。此外，抗HIV阳性患者中同时出现HDV和HBV复制的情况更为频繁（60%对18%，p<0.05），且水平更高。此外，在大多数抗HIV阳性受试者中，在一整年的随访期间，HDV RNA和HBV DNA始终呈阳性。
