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Isolation and primary structure of glucagon from the endocrine pancreas of Thunnus obesus.

作者信息

Navarro I, Gutiérrez J, Caixach J, Rivera J, Planas J

机构信息

Departament de Bioquímica i Fisiologia, Facultat de Biologia, Universitat de Barcelona, Spain.

出版信息

Gen Comp Endocrinol. 1991 Aug;83(2):227-32. doi: 10.1016/0016-6480(91)90025-2.

DOI:10.1016/0016-6480(91)90025-2
PMID:1916209
Abstract

Glucagon has been isolated from the endocrine pancreas of a tunid, Thunnus obesus. The primary structure of the glucagon molecule was established as H S E G T F S N D Y S K Y L E T R R A Q D F V Q W L K N S. The sequence is identical to those of sculpin and flounder glucagon and glucagon II from anglerfish. It also shows high homology to the mammalian hormone (76%). The mass determined by fast-atom bombardment (3508) was consistent with the proposed structure. Immunological properties of the tuna glucagon were analyzed by radioimmunoassay, showing a high degree of cross-reactivity with the 30K antibody.

摘要

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