雀鳝（匙吻鲟雀鳝）胰高血糖素、胃泌酸调节素和胰高血糖素样肽的分离：胃泌酸调节素和胰高血糖素样肽的氨基酸序列

Isolation of alligator gar (Lepisosteus spatula) glucagon, oxyntomodulin, and glucagon-like peptide: amino acid sequences of oxyntomodulin and glucagon-like peptide.

作者信息

Pollock H G, Kimmel J R, Ebner K E, Hamilton J W, Rouse J B, Lance V, Rawitch A B

机构信息

Department of Biochemistry, University of Kansas Medical Center, Kansas City 66103.

出版信息

Gen Comp Endocrinol. 1988 Jan;69(1):133-40. doi: 10.1016/0016-6480(88)90062-7.

DOI:10.1016/0016-6480(88)90062-7

PMID:3282974

Abstract

Oxyntomodulin, glucagon, and a glucagon-like peptide (GLP) have been isolated from the endocrine pancreas of the alligator gar (Lepisosteus spatula), a ganoid fish. The three peptides were isolated by gel filtration and HPLC and were identified by size, composition, and glucagon-like immunoreactivity. The amino acid sequences of the oxyntomodulin and GLP were determined. The oxyntomodulin contains 36 amino acid residues and its sequence is H S Q G T F T N D Y S K Y L D T R R A Q D F V Q W L M S T K R S G G I T. The composition of the glucagon is identical to the N-terminal 29 residues of the gar oxyntomodulin. The single form of GLP found contains 34 amino acid residues in the following sequence: H A D G T Y T S D V S S Y L Q D Q A A K K F V T W L K Q G Q D R R E. These findings suggest that all three peptides are derived from a common precursor.

摘要

胃泌酸调节素、胰高血糖素和一种胰高血糖素样肽（GLP）已从雀鳝（Lepisosteus spatula）（一种硬鳞鱼）的内分泌胰腺中分离出来。这三种肽通过凝胶过滤和高效液相色谱法分离，并通过大小、组成和胰高血糖素样免疫反应性进行鉴定。测定了胃泌酸调节素和GLP的氨基酸序列。胃泌酸调节素含有36个氨基酸残基，其序列为H S Q G T F T N D Y S K Y L D T R R A Q D F V Q W L M S T K R S G G I T。胰高血糖素的组成与雀鳝胃泌酸调节素的N端29个残基相同。所发现的单一形式的GLP含有34个氨基酸残基，序列如下：H A D G T Y T S D V S S Y L Q D Q A A K K F V T W L K Q G Q D R R E。这些发现表明，这三种肽都来自一个共同的前体。