Deshayes L, Levy D, Parodi A L, Levy J P
J Virol. 1977 Mar;21(3):1056-60. doi: 10.1128/JVI.21.3.1056-1060.1977.
The bovine leukemia virus (BLV) was purified from a chronically infected fetal lamb kidney cell line. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of this virus revealed the presence of eight distinguishable viral components with molecular weights ranging from 80,000 to 11,000. The major component is a non-glycosylated protein having a molecular weight of 24,000 (p24). At least three heavier polypeptides were found, one of them representing a glycoprotein (gp 60). In addition, four minor polypeptides with respective molecular weights of 19,000, 16,000, 13,000, and 11,000 were identified. In a complement fixation assay using naturally occurring antibodies of a leukemic cow, four polypeptides, which included gp 60, p35, p24, and p16, were found to be reactive.
牛白血病病毒（BLV）是从一个慢性感染的胎羊肾细胞系中纯化出来的。对该病毒进行的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析显示，存在八种可区分的病毒成分，其分子量范围为80,000至11,000。主要成分是一种分子量为24,000的非糖基化蛋白（p24）。发现了至少三种分子量更大的多肽，其中一种是糖蛋白（gp 60）。此外，还鉴定出了四种分子量分别为19,000、16,000、13,000和11,000的次要多肽。在使用白血病牛的天然抗体进行的补体结合试验中，发现包括gp 60、p35、p24和p16在内的四种多肽具有反应性。
