Rosić B, Sulović V, Juznić N, Lazarević B, Vidanović M, Milacić D
Glas Srp Akad Nauka Med. 1991(41):71-9.
The introduction deals with of the history, etiology and, briefly, clinical manifestations of endemic nephropathy. The authors state that a large number of investigations have been done until today, but without producing a diagnostic test for early diagnosis of the disease. This led the authors to pursue certain laboratory investigations. The control group consisted of 30 healthy individuals while patients diagnosed as Endemic Nephropathy were grouped according to stages of the disease: initial, developed, terminal stages. Nucleotides in erythrocytes (5' UMP, 5' IMP, 5' GMP, 5,CMP, 5' AMP, 5' ADP, 5' ATP) were determined on the Acidic Aminoanalyzer, with use of a special UV detector. Catecholamines in the urine were determined by using ratiofluorometery. Creatinine phosphocinase, hydroxybutirat--dehydrogenase and 2,3 DPG were determined on the "Carlbiochem" enzymometer. LDH, its isoenzymes as well as its profile were determined by electrophoresis on starch gel. Specific proteins such as haptoglobin, alpha 2 macroglobulin, alpha 1 antitrypsin, alpha 1 acid glycoprotein, properdin factor B, ceruloplasmin were determined on the Immunochemistry Analyzer. Total cholesterol and cholesterol in lipoproteins fractions VLDL, LDL and HDL were determined on the Cholesterol Analyzer. All results were evaluated statistically. Our conclusion is that they are interesting and contribute to the diagnosis of endemic nephropathy.
引言部分涉及地方性肾病的历史、病因，简要介绍了其临床表现。作者指出，截至目前已开展了大量调查，但尚未找到可用于该疾病早期诊断的诊断测试。这促使作者进行某些实验室研究。对照组由30名健康个体组成，而被诊断为地方性肾病的患者则根据疾病阶段进行分组：初期、进展期、终末期。使用特殊的紫外检测器，在酸性氨基酸分析仪上测定红细胞中的核苷酸（5'-UMP、5'-IMP、5'-GMP、5'-CMP、5'-AMP、5'-ADP、5'-ATP）。采用比率荧光测定法测定尿液中的儿茶酚胺。在“卡尔生物化学”酶标仪上测定磷酸肌酸激酶、羟丁酸脱氢酶和2,3-二磷酸甘油酸。通过淀粉凝胶电泳测定乳酸脱氢酶及其同工酶以及其图谱。在免疫化学分析仪上测定特异性蛋白质，如触珠蛋白、α2巨球蛋白、α1抗胰蛋白酶、α1酸性糖蛋白、备解素因子B、铜蓝蛋白。在胆固醇分析仪上测定总胆固醇以及极低密度脂蛋白、低密度脂蛋白和高密度脂蛋白等脂蛋白组分中的胆固醇。所有结果均进行统计学评估。我们的结论是，这些结果很有意思，有助于地方性肾病的诊断。
