在囊性纤维化中，胆碱能刺激未能诱导直肠黏膜产生分泌反应。
Failure of cholinergic stimulation to induce a secretory response from the rectal mucosa in cystic fibrosis.
作者信息
Hardcastle J, Hardcastle P T, Taylor C J, Goldhill J
机构信息
Department of Biomedical Science, The University, Sheffield.
出版信息
Gut. 1991 Sep;32(9):1035-9. doi: 10.1136/gut.32.9.1035.
Abstract
The secretory response to cholinergic stimulation was investigated in rectal biopsy specimens from children with cystic fibrosis and a control group using a modified Ussing chamber technique. Acetylcholine (10(-3) mol/l) increased the short circuit current in 12 control specimens by mean (SEM) 83.0 (16.4) microA/cm2, but samples from five children with cystic fibrosis failed to exhibit such a response (-1.4 (3.2) microA/cm2). Amiloride (10(-4) mol/l), which will inhibit electrogenic sodium absorption in viable tissues, caused similar reductions in the short circuit current of both control and cystic fibrosis tissues (control = -37.7 (7.7) microA/cm2; cystic fibrosis = -44.0 (9.3) microA/cm2). Thus, the failure of chloride secretion observed in the small intestine also exists in the rectal mucosa. This observation could be used both to aid diagnosis and to study the basic defect.
摘要
采用改良的尤斯灌流小室技术，对囊性纤维化患儿及对照组儿童的直肠活检标本中胆碱能刺激的分泌反应进行了研究。乙酰胆碱（10⁻³mol/L）使12个对照标本的短路电流平均（标准误）增加83.0（16.4）μA/cm²，但5例囊性纤维化患儿的标本未表现出这种反应（-1.4（3.2）μA/cm²）。氨氯吡咪（10⁻⁴mol/L）可抑制活组织中的电生性钠吸收，使对照组织和囊性纤维化组织的短路电流出现类似程度的降低（对照=-37.7（7.7）μA/cm²；囊性纤维化=-44.0（9.3）μA/cm²）。因此，在小肠中观察到的氯分泌障碍在直肠黏膜中也存在。这一观察结果可用于辅助诊断和研究基本缺陷。
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