在囊性纤维化中，胆碱能刺激未能诱导直肠黏膜产生分泌反应。
Failure of cholinergic stimulation to induce a secretory response from the rectal mucosa in cystic fibrosis.
作者信息
Hardcastle J, Hardcastle P T, Taylor C J, Goldhill J
机构信息
Department of Biomedical Science, The University, Sheffield.
出版信息
Gut. 1991 Sep;32(9):1035-9. doi: 10.1136/gut.32.9.1035.
Abstract
The secretory response to cholinergic stimulation was investigated in rectal biopsy specimens from children with cystic fibrosis and a control group using a modified Ussing chamber technique. Acetylcholine (10(-3) mol/l) increased the short circuit current in 12 control specimens by mean (SEM) 83.0 (16.4) microA/cm2, but samples from five children with cystic fibrosis failed to exhibit such a response (-1.4 (3.2) microA/cm2). Amiloride (10(-4) mol/l), which will inhibit electrogenic sodium absorption in viable tissues, caused similar reductions in the short circuit current of both control and cystic fibrosis tissues (control = -37.7 (7.7) microA/cm2; cystic fibrosis = -44.0 (9.3) microA/cm2). Thus, the failure of chloride secretion observed in the small intestine also exists in the rectal mucosa. This observation could be used both to aid diagnosis and to study the basic defect.
摘要
采用改良的尤斯灌流小室技术，对囊性纤维化患儿及对照组儿童的直肠活检标本中胆碱能刺激的分泌反应进行了研究。乙酰胆碱（10⁻³mol/L）使12个对照标本的短路电流平均（标准误）增加83.0（16.4）μA/cm²，但5例囊性纤维化患儿的标本未表现出这种反应（-1.4（3.2）μA/cm²）。氨氯吡咪（10⁻⁴mol/L）可抑制活组织中的电生性钠吸收，使对照组织和囊性纤维化组织的短路电流出现类似程度的降低（对照=-37.7（7.7）μA/cm²；囊性纤维化=-44.0（9.3）μA/cm²）。因此，在小肠中观察到的氯分泌障碍在直肠黏膜中也存在。这一观察结果可用于辅助诊断和研究基本缺陷。
相似文献
1
Failure of cholinergic stimulation to induce a secretory response from the rectal mucosa in cystic fibrosis.在囊性纤维化中，胆碱能刺激未能诱导直肠黏膜产生分泌反应。
Gut. 1991 Sep;32(9):1035-9. doi: 10.1136/gut.32.9.1035.
2
Ion transport abnormalities in rectal suction biopsies from children with cystic fibrosis.囊性纤维化患儿直肠吸引活检中的离子转运异常。
Gastroenterology. 1991 Aug;101(2):398-403. doi: 10.1016/0016-5085(91)90017-f.
3
Failure to induce secretion in jejunal biopsies from children with cystic fibrosis.对患有囊性纤维化儿童的空肠活检组织未能诱导出分泌物。
Gut. 1988 Jul;29(7):957-62. doi: 10.1136/gut.29.7.957.
4
Determinants of mild clinical symptoms in cystic fibrosis patients. Residual chloride secretion measured in rectal biopsies in relation to the genotype.囊性纤维化患者轻度临床症状的决定因素。直肠活检中测得的残余氯分泌与基因型的关系。
J Clin Invest. 1994 Feb;93(2):461-6. doi: 10.1172/JCI116993.
5
Colonic transepithelial potential difference in infants with cystic fibrosis.囊性纤维化婴儿的结肠跨上皮电位差
J Pediatr. 1991 Mar;118(3):412-5. doi: 10.1016/s0022-3476(05)82158-4.
6
In vivo evidence of altered chloride but not potassium secretion in cystic fibrosis rectal mucosa.囊性纤维化直肠黏膜中氯分泌改变而非钾分泌改变的体内证据。
Gastroenterology. 1991 Oct;101(4):1012-9. doi: 10.1016/0016-5085(91)90728-4.
7
Effect of amiloride on potential difference across rectal mucosa in cystic fibrosis patients.氨氯吡咪对囊性纤维化患者直肠黏膜跨膜电位差的影响。
Pediatr Res. 1982 Dec;16(12):1035-6. doi: 10.1203/00006450-198212000-00014.
8
Hyperabsorption of Na+ and raised Ca(2+)-mediated Cl- secretion in nasal epithelia of CF mice.囊性纤维化（CF）小鼠鼻上皮中Na⁺的过度吸收以及Ca²⁺介导的Cl⁻分泌增加。
Am J Physiol. 1994 May;266(5 Pt 1):C1478-83. doi: 10.1152/ajpcell.1994.266.5.C1478.
9
Defective cholinergic Cl(-) secretion and detection of K(+) secretion in rectal biopsies from cystic fibrosis patients.囊性纤维化患者直肠活检中胆碱能性氯离子分泌缺陷及钾离子分泌检测
Am J Physiol Gastrointest Liver Physiol. 2000 Apr;278(4):G617-24. doi: 10.1152/ajpgi.2000.278.4.G617.
10
Colonic and esophageal transepithelial potential difference in cystic fibrosis.囊性纤维化患者的结肠和食管跨上皮电位差
Gastroenterology. 1989 Apr;96(4):1041-8. doi: 10.1016/0016-5085(89)91621-1.
引用本文的文献
1
Potential of Intestinal Current Measurement for Personalized Treatment of Patients with Cystic Fibrosis.肠道电流测量在囊性纤维化患者个性化治疗中的潜力
J Pers Med. 2021 May 8;11(5):384. doi: 10.3390/jpm11050384.
2
Carbachol-induced colonic mucus formation requires transport via NKCC1, K⁺ channels and CFTR.卡巴胆碱诱导的结肠黏液形成需要通过NKCC1、钾离子通道和囊性纤维化跨膜传导调节因子进行转运。
Pflugers Arch. 2015 Jul;467(7):1403-1415. doi: 10.1007/s00424-014-1595-y. Epub 2014 Aug 21.
3
Toxin mediated diarrhea in the 21 century: the pathophysiology of intestinal ion transport in the course of ETEC, V. cholerae and rotavirus infection.二十一世纪的毒素介导性腹泻：肠毒素性大肠杆菌、霍乱弧菌和轮状病毒感染过程中的肠道离子转运的病理生理学。
Toxins (Basel). 2010 Aug;2(8):2132-57. doi: 10.3390/toxins2082132. Epub 2010 Aug 10.
4
Bestrophin-2 mediates bicarbonate transport by goblet cells in mouse colon.Bestrophin-2 介导小鼠结肠杯状细胞中的碳酸氢盐转运。
J Clin Invest. 2010 May;120(5):1722-35. doi: 10.1172/JCI41129. Epub 2010 Apr 19.
5
Effect of genistein on native epithelial tissue from normal individuals and CF patients and on ion channels expressed in Xenopus oocytes.金雀异黄素对正常个体和囊性纤维化患者的天然上皮组织以及非洲爪蟾卵母细胞中表达的离子通道的影响。
Br J Pharmacol. 2000 Aug;130(8):1884-92. doi: 10.1038/sj.bjp.0703520.
6
Electrophysiological characterization of human distal colon epithelium isolated using a novel technique.使用一种新技术分离的人远端结肠上皮的电生理特性
Dig Dis Sci. 1998 Nov;43(11):2439-45. doi: 10.1023/a:1026678030939.
7
In vitro bioelectric properties of bronchial epithelium from transplanted lungs in recipients with cystic fibrosis.囊性纤维化受体移植肺支气管上皮的体外生物电特性
Thorax. 1993 Oct;48(10):1006-11. doi: 10.1136/thx.48.10.1006.
8
Cellular chloride depletion inhibits cAMP-activated electrogenic chloride fluxes in HT29-18-C1 cells.细胞内氯离子耗竭抑制HT29-18-C1细胞中cAMP激活的电生性氯通量。
J Membr Biol. 1995 May;145(2):129-41. doi: 10.1007/BF00237371.
本文引用的文献
1
Absorption and secretion of fluid and electrolytes by the rectum.直肠对液体和电解质的吸收与分泌
Scand J Gastroenterol Suppl. 1984;93:79-87.
2
Ca2+ in the control of active intestinal Na and Cl transport: involvement in neurohumoral action.钙离子对肠道钠和氯主动转运的调控：参与神经体液作用。
Am J Physiol. 1983 Aug;245(2):G165-77. doi: 10.1152/ajpgi.1983.245.2.G165.
3
Accounting for cystic fibrosis.囊性纤维化的核算
Nature. 1988 Sep 15;335(6187):211. doi: 10.1038/335211a0.
4
Rectum has abnormal ion transport but normal cAMP-binding proteins in cystic fibrosis.
Am J Physiol. 1988 May;254(5 Pt 1):C719-24. doi: 10.1152/ajpcell.1988.254.5.C719.
5
Cystic fibrosis.囊性纤维化
J Clin Invest. 1987 Dec;80(6):1523-6. doi: 10.1172/JCI113237.
6
Effects of enteric neural stimulation on chloride transport in human left colon in vitro.肠神经刺激对人离体左结肠氯离子转运的影响。
Dig Dis Sci. 1989 Feb;34(2):206-13. doi: 10.1007/BF01536052.
7
Absence of secretory response in jejunal biopsy samples from children with cystic fibrosis.囊性纤维化患儿空肠活检样本中无分泌反应。
Lancet. 1987 Jul 11;2(8550):107-8. doi: 10.1016/s0140-6736(87)92781-4.
8
Failure to induce secretion in jejunal biopsies from children with cystic fibrosis.对患有囊性纤维化儿童的空肠活检组织未能诱导出分泌物。
Gut. 1988 Jul;29(7):957-62. doi: 10.1136/gut.29.7.957.
9
Altered intestinal chloride transport in cystic fibrosis.囊性纤维化中肠道氯化物转运的改变。
FASEB J. 1988 Jul;2(10):2625-9. doi: 10.1096/fasebj.2.10.2838365.
10
Ion transport and enteric nervous system (ENS) in rat rectal colon: mechanical stretch causes electrogenic Cl-secretion via plexus Meissner and amiloride-sensitive electrogenic Na-absorption is not affected by intramural neurons.大鼠直肠结肠中的离子转运与肠神经系统（ENS）：机械牵张通过迈斯纳丛引起电生性氯分泌，而氨氯地平敏感的电生性钠吸收不受壁内神经元影响。
Pflugers Arch. 1989 Jun;414(2):216-21. doi: 10.1007/BF00580966.
Zotero 插件