Department of Neuropsychiatry, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
J Hum Genet. 2009 Feb;54(2):122-6. doi: 10.1038/jhg.2008.17. Epub 2009 Jan 23.
Panic disorder (PD) is an anxiety disorder characterized by panic attacks and anticipatory anxiety. Although a number of association studies have been conducted, no gene has been identified as a susceptibility locus. In this study, we conducted a genome-wide association study of PD in 200 Japanese patients and the same number of controls, using the GeneChip Human Mapping 500 K Array Set. Genotypes were determined using the Bayesian Robust Linear Model with Mahalanobis (BRLMM) genotype calling algorithm. The genotype data were data-cleaned using criteria for SNP call rate (>or=95%), Hardy-Weinberg equilibrium (P>or=0.1%) and minor allele frequency (>or=5%). The significance level of the allele P-value was set at 1.0 x 10(-6), to make false discovery rate (FDR) <0.05. As a result, seven SNPs were significantly associated with PD, which were located in or adjacent to genes including PKP1, PLEKHG1, TMEM16B, CALCOCO1, SDK2 and CLU (or APO-J). Studies with other samples are required to confirm the results.
惊恐障碍（PD）是一种以惊恐发作和预期焦虑为特征的焦虑障碍。尽管已经进行了许多关联研究，但没有发现一个基因作为易感性基因座。在这项研究中，我们使用基因芯片人类图谱 500 K 数组集，对 200 名日本患者和相同数量的对照进行了 PD 的全基因组关联研究。使用贝叶斯稳健线性模型与马氏距离（BRLMM）基因型调用算法确定基因型。使用 SNP 调用率（>or=95%）、哈迪-温伯格平衡（P>or=0.1%）和次要等位基因频率（>or=5%）的标准对基因型数据进行数据清理。等位基因 P 值的显著水平设置为 1.0 x 10(-6)，以使错误发现率（FDR）<0.05。结果，七个 SNP 与 PD 显著相关，这些 SNP 位于 PKP1、PLEKHG1、TMEM16B、CALCOCO1、SDK2 和 CLU（或 APO-J）等基因内或附近。需要进行其他样本的研究来确认这些结果。
