N2'→p3'氨基磷酸甘油核酸作为一种潜在的替代遗传系统。

Chen Jesse J, Cai Xin, Szostak Jack W

Howard Hughes Medical Institute, and Department of Molecular Biology and Center for Computational and Integrative Biology, Massachusetts General Hospital, 185 Cambridge Street, Boston, Massachusetts 02114, USA.

J Am Chem Soc. 2009 Feb 18;131(6):2119-21. doi: 10.1021/ja809069b.

Glycerol nucleic acid (GNA) is an interesting base-pairing system with an acyclic, three-carbon backbone. In the present study, GNA analogues with N2'-->P3' phosphoramidate linkages (npGNA) have been synthesized and their base-pairing properties examined. Thermal denaturation and circular dichroism studies show that npGNA can form stable duplexes with itself and with GNA. Furthermore, we show that npGNA can be assembled by template-directed ligation of 3'-imidazole-activated-2'-amino GNA dinucleotides. These results suggest that npGNA is a potential candidate for a self-replicating system based upon phosphoramidate linkages.

甘油核酸（GNA）是一种有趣的碱基配对系统，其具有无环的三碳骨架。在本研究中，已合成了具有N2'→P3'氨基磷酸酯键的GNA类似物（npGNA），并对其碱基配对特性进行了研究。热变性和圆二色性研究表明，npGNA可以与自身以及与GNA形成稳定的双链体。此外，我们表明npGNA可以通过3'-咪唑活化的2'-氨基GNA二核苷酸的模板导向连接进行组装。这些结果表明，npGNA是基于氨基磷酸酯键的自我复制系统的潜在候选者。