Korde A, Meera V
Isotope Division, Bhabha Atomic Research Centre, Bombay, India.
Indian J Exp Biol. 1991 May;29(5):441-3.
Here we describe an enzymeimmunoassay (EIA) for thyroxine (T4) in serum, whose performance is comparable to that of a sensitive T4 radioimmunoassay (RIA). In this assay, specific T4 antibody adsorbed on polystyrene beads is used along with T4-horseradishperoxidase as the tracer and omicron-phenylenediamine as chromogen. Several samples were analysed both by this T4 EIA and by using a commercial RIA kit for T4. The results correlate well with a correlation coefficient r = 0.9 and slope = 0.93 (n = 50).
在此，我们描述了一种用于测定血清中甲状腺素（T4）的酶免疫测定法（EIA），其性能与灵敏的T4放射免疫测定法（RIA）相当。在该测定法中，吸附在聚苯乙烯珠上的特异性T4抗体与T4 - 辣根过氧化物酶作为示踪剂以及邻苯二胺作为显色剂一起使用。通过这种T4 EIA和使用市售的T4 RIA试剂盒对多个样本进行了分析。结果具有良好的相关性，相关系数r = 0.9，斜率 = 0.93（n = 50）。
